Results 81 to 90 of about 76,691 (283)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Simulation study of controlled atmosphere storage environment for sweet potatoes using modular turnover boxes

Shipin yu jixie
Objective: To mitigate unfavorable factors during the packing, transportation, and storage of fruits and vegetables, a foldable, preassembled turnover box was designed based on the premise of recycling. Methods: Standardized boxes were used to hold sweet
HU Rusheng, LIU Yin, MENG Zhaofeng, ZHANG Fan, WU Feijun   +4 more
doaj   +1 more source

Grain aeration in hangar storage by low-pressure ventilators

Research in Agricultural Engineering, 2005
In the paper are presented measured values of air output velocity from the stored grain layer in hangar storage within its aeration by low-pressure ventilators.
P. Kroupa, J. Skalický, P. Kovaříček
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Caracterização e modelagem multivariada do desempenho de sistemas de arquivos paralelos [PDF]

, 2015
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Ciência da Computação, Florianópolis, 2015.A quantidade de dados digitais gerados diariamente vem aumentando de forma significativa.
Inacio, Eduardo Camilo
core  

Spatial and temporal evolution of injected CO2 at the Sleipner Field, North Sea [PDF]

, 2012
Time-lapse, three-dimensional (3D) seismic surveys have imaged an accumulation of injected CO2 adjacent to the Sleipner field in the North Sea basin.
Chadwick, R. A., Boait, F.C., Huppert, H. E., Neufeld, J.A., White, N. J., Bickle, M.J., Boait, F. C., Chadwick, R.A., Huppert, H.E., Neufeld, J. A., Bickle, M. J., White, N.J., Boait, F.   +12 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Efek Lama Penyimpanan Semen Beku Sapi Bali pada Pos Inseminasi Buatan terhadap Membran Plasma, Tudung Akrosom Utuh, dan DNA Spermatozoa

Jurnal Ilmu Peternakan Terapan, 2020
The purpose of this study was to determine the effect of frozen storage time for Bali Bull in artificial insemination station in Samarinda City, East Kalimantan on the quality of motility, viability, velocity, abnormality, plasma membrane integrity (MIn)
Fikri Ardhani, Hayatul Mufidah, Rahmah Samsuriati, Hilman Pratama Putra   +3 more
doaj  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source