Results 41 to 50 of about 574,511 (219)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Particle Filter-Based Video Object Tracking Scheme With Target Remodeling and Reinitialization and Its Hardware Implementation Using Raspberry Pi

open access: yesIEEE Access
Video object tracking in real-world scenarios is one of the challenging problems of computer vision. The issue is compounded in the presence of varying illumination conditions, dynamic entities of the background, and bad weather conditions.
Jyotiranjan Panda   +2 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

Constructing a Gaming Model for Professional Tennis Players Using the C5.0 Algorithm

open access: yesApplied Sciences, 2022
Professional tennis players have their own habits of tactics and play. However, players’ shortcomings can be corrected by constantly practicing professional techniques and by tactical analysis.
Che-Wei Chang, Yu-Ran Qiu
doaj   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

LPDi GAN: A License Plate De-Identification Method to Preserve Strong Data Utility

open access: yesSensors
License plate (LP) information is an important part of personal privacy, which is protected by law. However, in some publicly available transportation datasets, the LP areas in the images have not been processed.
Xiying Li   +5 more
doaj   +1 more source

Behavioural Responses of Defended and Undefended Prey to Their Predator—A Case Study of Rotifera

open access: yesBiology, 2022
Predation is a strong species interaction causing severe harm or death to prey. Thus, prey species have evolved various defence strategies to minimize predation risk, which may be immediate (e.g., a change in behaviour) or transgenerational ...
Victor Parry   +3 more
doaj   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

open access: yesFEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho   +4 more
wiley   +1 more source

Novel Method to Conduct Remote Sensory Sessions and Biometrics during Isolation

open access: yesBiology and Life Sciences Forum, 2021
Background: The recent pandemic due to the appearance of COVID-19 in 2020 has led to lockdowns worldwide, which have affected companies and universities conducting sensory evaluations. Therefore, a novel method to conduct sensory sessions for descriptive
Claudia Gonzalez Viejo   +2 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

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