Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Adaptive Video Encoding for Different Video Codecs
By 2022, we expect video traffic to reach 82% of the total internet traffic. Undoubtedly, the abundance of video-driven applications will likely lead internet video traffic percentage to a further increase in the near future, enabled by associate ...
G. Esakki +3 more
doaj +1 more source
Full-reference video quality metric assisted the development of no-reference bitstream video quality metrics for real-time network monitoring [PDF]
High-quality video is being increasingly delivered over Internet Protocol networks, which means that network operators and service providers need methods to measure the quality of experience (QoE) of the video services. In this paper, we propose a method
Andreas Aurelius +12 more
core +2 more sources
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Object-Based Approach for Adaptive Source Coding of Surveillance Video
Intelligent analysis of surveillance videos over networks requires high recognition accuracy by analyzing good-quality videos that however introduce significant bandwidth requirement.
Tung-Ming Pan +2 more
doaj +1 more source
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell +8 more
wiley +1 more source
Fast implementations of block motion estimation algorithms in video encoders [PDF]
This research is aimed at designing and implementing novel fast algorithms for speeding up the encoding process. The objective of this study was to come up with algorithms which can estimate the data significantly faster than the existing algorithms ...
Koduri, Naga Rohini
core +1 more source
Perceived Quality of Full HD Video - Subjective Quality Assessment
In recent years, an interest in multimedia services has become a global trend and this trend is still rising. The video quality is a very significant part from the bundle of multimedia services, which leads to a requirement for quality assessment in the ...
Juraj Bienik +3 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A nationwide student survey on biochemistry learning resources in Italy
We conducted a survey among Italian university students to investigate their preferred study materials for biochemistry. We then analyzed the collected data to identify these materials. This information may be valuable to the Biochemistry educational community. The graphical abstract was created with Claude (Opus 5.5; Anthropic, 2026).
Anna Marabotti +4 more
wiley +1 more source

