Results 11 to 20 of about 188 (80)

Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome [PDF]

Virology Journal, 2008
Background Enteritis is caused by a spectrum of viruses that is most likely not fully characterised. When testing stool samples by cell culture, virus isolates are sometimes obtained which cannot be typed by current methods. In this study we used VIDISCA,
Luciano Kleber de Souza Luna, Marcus Panning, Sigrid Baumgarte   +2 more
exaly   +4 more sources

Autologous antibody capture to enrich immunogenic viruses for viral discovery. [PDF]

PLoS ONE, 2013
Discovery of new viruses has been boosted by novel deep sequencing technologies. Currently, many viruses can be identified by sequencing without knowledge of the pathogenicity of the virus. However, attributing the presence of a virus in patient material
Bas B Oude Munnink, Seyed Mohammad Jazaeri Farsani, Martin Deijs, Jiri Jonkers, Joost T P Verhoeven, Margareta Ieven, Herman Goossens, Menno D de Jong, Ben Berkhout, Katherine Loens, Paul Kellam, Margreet Bakker, Marta Canuti, Matthew Cotten, Lia van der Hoek   +14 more
doaj   +5 more sources

Identification and characterization of unrecognized viruses in stool samples of non-polio acute flaccid paralysis children by simplified VIDISCA [PDF]

Virology Journal, 2014
Background: The use of sequence independent methods combined with next generation sequencing for identification purposes in clinical samples appears promising and exciting results have been achieved to understand unexplained infections.
Salmaan Sharif, Marta Canuti, Shahzad Shaukat   +2 more
exaly   +6 more sources

Viral Metagenomics on Cerebrospinal Fluid [PDF]

Genes, 2019
Identifying the causative pathogen in central nervous system (CNS) infections is crucial for patient management and prognosis. Many viruses can cause CNS infections, yet screening for each individually is costly and time-consuming.
Matthijs Brouwer, Arthur W D Edridge, Cormac Kinsella   +2 more
exaly   +3 more sources

Random PCR and ultracentrifugation increases sensitivity and throughput of VIDISCA for screening of pathogens in clinical specimens

Journal of Infection in Developing Countries, 2010
Introduction: Virus discovery based on cDNA-AFLP (VIDISCA) is a sequence-independent virus discovery method that was recently developed and successfully used to characterize unknown viruses in cell cultures. Its applicability, however, is limited by its
Le Van Tan, H. Rogier van Doorn, Lia van der Hoek, Vo Minh Hien, Maarten F. Jebbink, Do Quang Ha, Jeremy Farrar, Nguyen Van Vinh Chau, Menno D. de Jong   +8 more
doaj   +2 more sources

Investigating an agnostic metagenomic virus discovery tool and evaluating the performance of Ion Torrent versus Oxford Nanopore Technology sequencing

International Journal of Infectious Diseases
Introduction and motivation: Emerging and re-emerging viruses are a global health concern. Historically, such viruses have been identified through targeted approaches, which has been the gold standard in diagnosis.
Ms Tshegofatso Mahlangu, Dr Tongai Maponga, Mr Martin Deijs, Prof Lia van der Hoek, Prof Gert van Zyl, Prof Davey Smith   +5 more
doaj   +2 more sources

Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses [PDF]

Influenza and Other Respiratory Viruses, 2015
Currently, virus discovery is mainly based on molecular techniques. Here, we propose a method that relies on virus culturing combined with state-of-the-art sequencing techniques.
Margareta Ieven, Ronald Dijkman, Rienk Jeeninga   +2 more
exaly   +3 more sources

Two novel parvoviruses in frugivorous New and Old World bats. [PDF]

PLoS ONE, 2011
Bats, a globally distributed group of mammals with high ecological importance, are increasingly recognized as natural reservoir hosts for viral agents of significance to human and animal health.
Marta Canuti, Anna Maria Eis-Huebinger, Martin Deijs, Michel de Vries, Jan Felix Drexler, Samuel K Oppong, Marcel A Müller, Stefan M Klose, Nele Wellinghausen, Veronika M Cottontail, Elisabeth K V Kalko, Christian Drosten, Lia van der Hoek   +12 more
doaj   +1 more source

Unexplained diarrhoea in HIV-1 infected individuals. [PDF]

BMC Infect Dis, 2014
Gastrointestinal symptoms, in particular diarrhoea, are common in non-treated HIV-1 infected individuals. Although various enteric pathogens have been implicated, the aetiology of diarrhoea remains unexplained in a large proportion of HIV-1 infected ...
Oude Munnink BB, Canuti M, Deijs M, de Vries M, Jebbink MF, Rebers S, Molenkamp R, van Hemert FJ, Chung K, Cotten M, Snijders F, Sol CJ, van der Hoek L.   +12 more
europepmc   +3 more sources

New Adenovirus in Bats, Germany

Emerging Infectious Diseases, 2009
We tested 55 deceased vespertilionid bats of 12 species from southern Germany for virus infections. A new adenovirus was isolated from tissue samples of 2 Pipistrellus pipistrellus bats, which represents the only chiropteran virus isolate found in Europe
Michael Sonntag, Kristin Mühldorfer, Stephanie Speck, Gudrun Wibbelt, Andreas Kurth   +4 more
doaj   +1 more source