Results 21 to 30 of about 10,290 (225)

Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Underlying Heterosis in Kenaf (Hibiscus Cannabinus L.) Drought Tolerance

Journal of Natural Fibers, 2022
Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop with significant heterosis and tolerance to abiotic stresses. However, the mechanism underlying heterosis in kenaf under drought conditions is completely unknown.
Dengjie Luo, Shan Cao, Zengqiang Li, Meiqiong Tang, Caijin Wang, Enerand Mackon, Zhen Huang, Jiao Pan, Xia Wu, Qijing Wu, Hui Zhang, Renxue Li, Xin Li, Ru Li, Peng Chen   +14 more
doaj   +1 more source

Silencing of SlDRB1 gene reduces resistance to tomato yellow leaf curl virus (TYLCV) in tomato (Solanum lycopersicum)

Plant Signaling & Behavior, 2022
Double-stranded RNA-binding proteins are small molecules in the RNA interference (RNAi) pathway that form the RNAi machinery together with the Dicer-like protein (DCL) as a cofactor.
Xin Huang, Jianming Wei, Dan Wu, Na Mi, Sili Fang, Yao Xiao, Yunzhou Li   +6 more
doaj   +1 more source

Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus

Horticultural Plant Journal, 2021
Virus-induced gene silencing (VIGS) is a genetic tool used to assess gene function. Tobacco rattle virus (TRV) is a VIGS vector commonly used to induce endogenous gene silencing in plants.
Chengyan Deng, Fan Zhang, Jiaying Wang, Yanfei Li, He Huang, Silan Dai   +5 more
doaj   +1 more source

Tobacco rattle virus-induced PHYTOENE DESATURASE (PDS) and Mg-chelatase H subunit (ChlH) gene silencing in Solanum pseudocapsicum L. [PDF]

PeerJ, 2018
Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. The present study constitutes the first application of VIGS in S. pseudocapsicum, which has great ornamental and pharmaceutical value, using tobacco rattle
Hua Xu, Leifeng Xu, Panpan Yang, Yuwei Cao, Yuchao Tang, Guoren He, Suxia Yuan, Jun Ming   +7 more
doaj   +2 more sources

Loss of Foreign DNA Inserts from Barley Stripe Mosaic Virus Vectors—Potential Consequences for Use in Functional Genomics Studies

International Journal of Plant Biology, 2023
Barley stripe mosaic virus (BSMV)-based vectors are frequently used in virus-induced gene silencing (VIGS) and, more recently, viral overexpression (VOX) studies in wheat.
Steven R. Scofield
doaj   +1 more source

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
doaj   +1 more source

A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS) [PDF]

, 2019
Virus-induced gene silencing (VIGS) is a rapid and cost-effective reverse genetic technology that can be used to assess gene function in wheat. This chapter contains a detailed description of how to target wheat meiotic genes by VIGS.
Kanyuka, K., Kostya Kanyuka, Stuart Desjardins, Higgins, J. D., Desjardins, S., James D. Higgins, Kanyuka, Kostya   +6 more
core   +1 more source

Virus-induced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants

Frontiers in Plant Science, 2014
Virus-induced gene silencing (VIGS) is an effective tool for gene function analysis in plants. Over the last decade, VIGS has been successfully used as both a forward and reverse genetics technique for gene function analysis in various model plants, as ...
Venkategowda eRamegowda, Kirankumar S Mysore, Muthappa eSenthil-Kumar   +2 more
doaj   +1 more source

Virus-Induced Gene Silencing (VIGS) in Cassava Using Geminivirus Agroclones.

, 2020
peer reviewedVirus-induced gene silencing (VIGS) is an efficient, low-cost, and rapid functional validation tool for candidate genes in planta. The VIGS approach is particularly suitable to perform reverse genetics studies in crop species.
Kumar Vasudevan, Ezequiel Matias Lentz, Vanderschuren, Hervé, Hervé Vanderschuren, Syed-Shan-e-Ali, Zaïdi, Lentz, Ezequiel Matias, Vasudevan, Kumar, Syed Shan-e-Ali Zaidi, Zaidi, Syed Shan-e-Ali, Matias Lentz, Ezequiel   +9 more
core   +1 more source

Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance [PDF]

, 2015
Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe
Rudd, J. J., Kanyuka, K., Lee, W-S., Kanyuka, Kostya, Rudd, Jason   +4 more
core   +1 more source