Results 41 to 50 of about 10,290 (225)

Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum

Plant Methods, 2020
Background Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics.
Ying Tan, Alfredas Bukys, Attila Molnár, Andrew Hudson   +3 more
doaj   +1 more source

Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Plant Methods, 2012
Background We previously developed a virus-induced gene silencing (VIGS) vector for cotton from the bipartite geminivirusCotton leaf crumple virus (CLCrV). The original CLCrV VIGS vector was designed for biolistic delivery by a gene gun.
Tuttle John, Haigler Candace H, Robertson Dominique   +2 more
doaj   +1 more source

HIV and me: The perceptions of children aged 10–12 living with HIV, and their expectations for adulthood

Koers : Bulletin for Christian Scholarship, 2020
This article explores the understanding that children living with HIV have of their condition, and the physical and psychosocial challenges they face in pursuit of their ideals for adulthood.
Hettie Van der Merwe, Alva Van der Merwe
doaj   +1 more source

Herbivory‐Induced Stomatal Opening Triggers the Dispersal of an Invasive Insect Herbivore

Advanced Science, EarlyView.
The invasive leafminer Phthorimaea absoluta uniquely manipulates plant defenses by inducing stomatal opening in tomato. This response, mediated by the stomatal regulator IQM1, enhances the release of the repellent volatile benzaldehyde. This volatile triggers the dispersal of adult moths, facilitates colonization of new hosts, and leads to rapid ...
Limin Chen, Xiaowei Li, Yaru Wang, Farman Ullah, Shuxing Zhou, Lemeng Dong, Weizhong He, Harro J. Bouwmeester, Youming Hou, Ian T. Baldwin, Yaobin Lu   +10 more
wiley   +1 more source

Knocking down wheat meiotic genes through Virus-induced gene-silencing (VIGS)

, 2019
Virus-induced gene-silencing (VIGS) is a reverse genetic technology that can be used to assess gene function in model and crop species. The technique exploits a natural viral defence response in plants, whereby invading viral RNAs are detected, and this ...
Kanyuka, K., Higgins, J. D., Desjardins, S.   +2 more
core   +2 more sources

Unveiling the role of the TIR1/AFB gene family in Capsicum annuum: genome-wide identification, characterization, and transcriptomic analysis under Ralstonia solanacearum infection

Vegetable Research
TIR1/AFB proteins play key roles in plants responding to Solanum crop disease. In this study, five putative TIR1/AFB proteins were identified in pepper (Capsicum annuum). Chromosomal mapping revealed that CaTIR1/AFB genes are located on chromosomes DH02,
Wenchao Du, Zhiyu Yu, Xiaoyan Miao, Zhongcai Teng, Chao Zhang, Bingqian Zhang, Meng Xia, Jingbin Hua, Umer Karamat, Peiqing Liu, Zhenhua Jia   +10 more
doaj   +1 more source

California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

BMC Plant Biology, 2021
Background Tobacco rattle virus (TRV) based virus-induced gene silencing (VIGS), a widely used functional genomics tool, requires growth temperatures typically lower than those of the plant’s native environment.
Jamilur Rahman, Ian T. Baldwin, Klaus Gase   +2 more
doaj   +1 more source

The Mitogen‐Activated Protein Kinase GhNTF3 Modulates the Arbuscular Mycorrhizal Symbiosis‐Immunity Trade‐Off in Cotton by Regulating Salicylic Acid Biosynthesis

Advanced Science, EarlyView.
AM symbiosis induces GhWAK13 to promote fungal colonization. GhNTF3 negatively regulates symbiosis by promoting SA biosynthesis. GhWAK13 sequesters GhNTF3 at the membrane, limiting its nuclear translocation and relieving SA‐mediated suppression. Under Verticillium stress, GhNTF3 enters the nucleus and interacts with GhJAZ6 to activate SA signaling ...
Shuangjie Jia, Jingshang Wen, Jihang Li, Meina Feng, Yiming He, Ruojun Liu, Qian Liu, Junli Zhang, Kai Cheng, Xiangyu Zhang, Xiao Zhang   +10 more
wiley   +1 more source

Immunofluorescence analysis of the FtsZ-ring in VIGS plants.

, 2013
Immunofluorescence images of the FtsZ2-1 ring of chloroplasts in VIGS-GFP plants and nongreen plastids in VIGS-ALB3 plants two weeks after infection. Chloroplasts are indicated by chlorophyll autofluorescence (red). Scale bars = 10 µm.
Dongru Feng (326335), Jianbin Su (326328), Jie Zhang (64655), Bing Liu (70818), Jun Liu (42548), Hongbin Wang (281658), Jinfa Wang (326337), Peng Wang (34436)   +7 more
core   +1 more source

An Effector TaCRVP From Tuta Absoluta Oral Secretion Impairs H2O2‐Mediated Plant Defense by Targeting Tomato SlCAT2

Advanced Science, EarlyView.
The Tuta absoluta salivary effector TaCRVP subverts tomato immunity by hijacking host catalase SlCAT2. Through a sophisticated dual mechanism, TaCRVP simultaneously promotes SlCAT2 tetramerization for activation and blocks its SlAdBiL‐mediated degradation for stabilization.
Jianquan Yan, Youdan Zhang, Zhujun Wang, Huatao Tang, Huiping Liu, Zi Chen, Shuai Liu, Jiayi Shi, Lu Peng, Youming Hou   +9 more
wiley   +1 more source