Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis [PDF]
Nature Communications, 2020 Bacillus thuringiensis Vip3 (Vegetative Insecticidal Protein 3) toxins are widely used in biotech crops to control Lepidopteran pests. Here authors present the cryo-EM structures of the protoxin and the protease-activated state of Vip3Aa which shed light
Rafael Núñez-Ramírez +5 more
doaj +5 more sources
Receptor interactions of protoxin and activated Vip3Aa structural conformations in Spodoptera exigua
Pest Management Science, Volume 80, Issue 12, Page 6142-6149, December 2024.In vitro assays using mutants of Vip3Aa show that both protoxin and activated conformations bind to the same receptors in the S. exigua midgut. In vivo assays confirm the binding functionality of both forms. Abstract BACKGROUND The Vip3Aa insecticidal protein, produced by Bacillus thuringiensis, has been effectively used in commercial Bt‐crops to ...
Maria Lázaro‐Berenguer +2 more
wiley +4 more sources
Control Efficacy of the Bt Maize Event DBN3601T Expressing Cry1Ab and Vip3Aa Proteins against Beet Armyworm, Spodoptera exigua (Hübner), in China [PDF]
PlantsThe beet armyworm, Spodoptera exigua (Hübner), is a major pest of maize, cotton, soybean, and many other crops globally. Despite the widespread deployment of Bt transgenic maize for pest control worldwide, the efficacy of Bt lepidopteran-resistant ...
Cheng Song +4 more
doaj +2 more sources
Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony [PDF]
Toxins, 2020 The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests.
Daniel Pinos +6 more
doaj +6 more sources
In vivo competition assays between Vip3 proteins confirm the occurrence of shared binding sites in Spodoptera littoralis [PDF]
Scientific Reports, 2022 Due to their different specificity, the use of Vip3 proteins from Bacillus thuringiensis in combination with the conventionally used Cry proteins in crop protection is being essential to counteract the appearance of insect resistance.
María Lázaro-Berenguer +3 more
doaj +2 more sources
Insights into the Structure of the Vip3Aa Insecticidal Protein by Protease Digestion Analysis [PDF]
Toxins, 2017 Vip3 proteins are secretable proteins from Bacillus thuringiensis whose mode of action is still poorly understood. In this study, the activation process for Vip3 proteins was closely examined in order to better understand the Vip3Aa protein stability and
Yolanda Bel +4 more
doaj +3 more sources
PLoS ONE, 2022 The gram pod borer is a major pest of chickpea, accounting for average annual yield losses to the tune of 40-50%. VIP3Aa, a class of insecticidal protein with different receptor binding site in the insect's midgut compared to Bt-crystal protein, offers ...
Prateek Singh +6 more
doaj +3 more sources
In-silico determination of insecticidal potential of Vip3Aa-Cry1Ac fusion protein against Lepidopteran targets using molecular docking [PDF]
Frontiers in Plant Science, 2015 Study and research of Bt (Bacillus thuringiensis) transgenic plants have opened new ways to combat insect pests. Over the decades, however, insect pests, especially the Lepidopteran, have developed tolerance against Bt delta-endotoxins.
Aftab eAhmad +7 more
doaj +3 more sources
Evaluation of GS-omega/kappa-Hxtx-Hv1a and Bt toxins against Bt-resistant and -susceptible strains of Helicoverpa zea (Boddie) and Spodoptera frugiperda (J.E. Smith). [PDF]
Pest Manag SciThe addition of 10 μg cm−2 of Cry1Ac to the surface of GS‐omega/kappa‐Hxtx‐Hv1a (ω/κ‐Hv1a) incorporated diet can significantly increase the mortality of Cry‐resistant Helicoverpa zea compared to diets without the Cry1Ac toxin. Abstract BACKGROUND Helicoverpa zea and Spodoptera frugiperda are destructive insect pests of cotton and corn.
Ross S +8 more
europepmc +2 more sources
Biophysical Analysis of Vip3Aa Toxin Mutants Before and After Activation. [PDF]
Int J Mol SciCry toxins from Bacillus thuringiensis are effective biopesticides that kill lepidopteran pests, replacing chemical pesticides that indiscriminately attack both target and non-target organisms. However, resistance in susceptible pests is an emerging problem. B.
Khunrach P +4 more
europepmc +4 more sources