Results 141 to 150 of about 4,157,307 (334)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

The development of a lateral flow immunochromatographic test strip for measurement of specific IgA and IgG antibodies level against porcine epidemic diarrhea virus in pig milk

Veterinary Quarterly
Porcine epidemic diarrhea virus (PEDV) causes severe enteric disease and high mortality in neonatal piglets, leading to significant economic losses in the swine industry. Considering that passive lactogenic immunity is crucial for preventing infection in
Patumporn Jermsutjarit, Dhithya Venkateswaran, Nitaya Indrawattana, Jessada Na Plord, Angkana Tantituvanont, Dachrit Nilubol   +5 more
doaj   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Author Correction: Intradermal needle-free injection prevents African Swine Fever transmission, while intramuscular needle injection does not

Scientific Reports, 2023
Muhammad Salman, Hongyao Lin, Roypim Suntisukwattana, Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol   +6 more
doaj   +1 more source

Influenza Hemagglutinin and Neuraminidase: Yin–Yang Proteins Coevolving to Thwart Immunity

Viruses, 2019
Influenza A virions possess two surface glycoproteins—the hemagglutinin (HA) and neuraminidase (NA)—which exert opposite functions. HA attaches virions to cells by binding to terminal sialic acid residues on glycoproteins/glycolipids to ...
Ivan Kosik, Jonathan W. Yewdell
doaj   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Giant Viruses—Big Surprises

Viruses, 2019
Viruses are the most prevalent infectious agents, populating almost every ecosystem on earth. Most viruses carry only a handful of genes supporting their replication and the production of capsids.
Nadav Brandes, Michal Linial
doaj   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Virus Replication as a Phenotypic Version of Polynucleotide Evolution

, 2013
In this paper we revisit and adapt to viral evolution an approach based on the theory of branching process advanced by Demetrius, Schuster and Sigmund ("Polynucleotide evolution and branching processes", Bull. Math. Biol.
A. Eyre-Walker, A. Kowald, A. Timm, C. Escarmís, C. J. Thompson, C. Meyer, C. O. Wilke, C. O. Wilke, D. A. Steinhauer, D. R. Rokyta, Diogo Castro, E. Domingo, E. Domingo, E. Lázaro, F. Antoneli, F. Antoneli, F. Gantmacher, Fernando Antoneli, Francisco Bosco, H. A. Orr, H. W. Watson, I. Leuthäusser, J. A. Capitán, J. A. Cuesta, J. A. Cuesta, J. Aguirre, J. Aguirre, J. H. Wilkinson, J. Hermisson, J. J. Bull, J. J. Bull, J. J. Bull, J. Swetina, K. B. Athreya, K. Dietz, L. Demetrius, L. Demetrius, Luiz Mario Janini, M. Delbrück, M. Eigen, M. Eigen, M. Imhof, M. Jiřina, M. Kimmel, M. Parera, M. Ziehe, P. Carrasco, P. D. Keightley, P. Domingo-Calap, P. Jagers, R. Forster, R. M. Gray, R. Miralles, R. Sanjuán, S. C. Manrubia, S. Crotty, S. F. Elena, S. F. Elena, S. Ojosnegros, S. Ojosnegros, T. G. Kurtz, T. Harris, U. Grenander, W. Feller, Y. Zhu   +64 more
core   +1 more source