Results 81 to 90 of about 259,105 (298)
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Viruses, 2014 Ebola virus (EBOV) causes viral hemorrhagic fever in humans and can have clinical fatality rates of ~60%. The EBOV genome consists of negative sense RNA that encodes seven proteins including viral protein 40 (VP40).
Emmanuel Adu-Gyamfi +5 more
doaj +1 more source
The Nef protein of human immunodeficiency virus type 1 enhances serine phosphorylation of the viral matrix [PDF]
Journal of Virology, 1997 The human immunodeficiency virus type 1 matrix (MA) protein is phosphorylated during virion maturation on its C-terminal tyrosine and on several serine residues. Whereas MA tyrosine phosphorylation facilitates viral nuclear import, the significance of MA serine phosphorylation remains unclear.
S, Swingler +5 more
openaire +2 more sources
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
FEBS Open Bio, EarlyView.Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
wiley +1 more source
A novel biclustering approach to association rule mining for predicting HIV-1-human protein interactions. [PDF]
PLoS ONE, 2012 Identification of potential viral-host protein interactions is a vital and useful approach towards development of new drugs targeting those interactions.
Anirban Mukhopadhyay +2 more
doaj +1 more source
Inhibition of Banzi Viral RNA Synthesis in BHK-21 Cells by Banzi Virion Matrix Protein
Intervirology, 1986 Treatment of BHK-21 cells with purified matrix (M) protein isolated from Banzi virions resulted in decreased production of Banzi virus and in reduced levels of virus-specific positive-strand and negative-strand RNA. The M protein had no effect on the uncoating of the viral genome.
G A, Hommel-Berrey, R H, Schloemer
openaire +2 more sources
FEBS Open Bio, EarlyView.KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani +6 more
wiley +1 more source
Protein intrinsic disorder toolbox for comparative analysis of viral proteins
BMC Genomics, 2008 To examine the usefulness of protein disorder predictions as a tool for the comparative analysis of viral proteins, a relational database has been constructed. The database includes proteins from influenza A and HIV-related viruses.
Uversky Vladimir N +2 more
doaj +1 more source
HIV-1 Virus-like Particle Budding Assay
Bio-Protocol, 2013 Viral replication culminates with the egress of the mature virion from the host cell. This step of the viral life cycle has recently garnered increased attention with the discovery of the cellular restriction factor, Tetherin, which tethers budded ...
Nathan Vande Burgt +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source