Results 81 to 90 of about 664,008 (371)

Efficient isolation on Vero.DogSLAMtag cells and full genome characterization of Dolphin Morbillivirus (DMV) by next generation sequencing [PDF]

, 2018
The Dolphin Morbillivirus (DMV) genome from the frst Mediterranean epidemic (1990-\u201992) is the only cetacean Morbillivirus that has been completely sequenced.
Acutis, Pier Luigi, Biolatti, Cristina, Caruso, Claudio, Casalone, Cristina, Cerutti, Francesco, Giorda, Federica, Grattarola, Carla, Masoero, Loretta, Mazzariol, Sandro, Mignone, Walter, Modesto, Paola, Pautasso, Alessandra, Peletto, Simone   +12 more
core   +2 more sources

NEDD4 family ubiquitin ligases associate with LCMV Z's PPXY domain and are required for virus budding, but not via direct ubiquitination of Z.

PLoS Pathogens, 2019
Viral late domains are used by many viruses to recruit the cellular endosomal sorting complex required for transport (ESCRT) to mediate membrane scission during viral budding.
Christopher M Ziegler, Loan Dang, Philip Eisenhauer, Jamie A Kelly, Benjamin R King, Joseph P Klaus, Inessa Manuelyan, Ethan B Mattice, David J Shirley, Marion E Weir, Emily A Bruce, Bryan A Ballif, Jason Botten   +12 more
doaj   +1 more source

Cellular liquid biopsy provides unique chances for disease monitoring, preclinical model generation and therapy adjustment in rare salivary gland cancer patients

Molecular Oncology, EarlyView.
We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić, Florian Lüke, Steffi Treitschke, Andrea Coluccio, Martin Hoffmann, Giancarlo Feliciello, Adithi Ravikumar Varadarajan, Xin Lu, Kathrin Weidele, Catherine Botteron, Silvia Materna–Reichelt, Felix Keil, Katja Evert, Florian Weber, Thomas Schamberger, Michael Althammer, Jirka Grosse, Dirk Hellwig, Christian Schulz, Stephan Seitz, Peter Ugocsai, Anke Schlenska‐Lange, Roman Mayr, Ulrich Kaiser, Wolfgang Dietmaier, Bernhard Polzer, Jens Warfsmann, Kamran Honarnejad, Tobias Pukrop, Daniel Heudobler, Christoph A. Klein, Christian Werno   +31 more
wiley   +1 more source

HIV-1 Structural Proteins or Cell-Signaling Factors? That Is the Question!

Current Issues in Molecular Biology
The biological activity of structural HIV-1 proteins is not limited to ensuring a productive viral infection but also interferes with cellular homeostasis through intra- and extracellular signaling activation.
Michele Pellegrino, Francesca Giordano, Francesca De Amicis, Maria Marra, Paola Tucci, Stefania Marsico, Stefano Aquaro   +6 more
doaj   +1 more source

Obtaining and Immunologic Characterization of Filoviruses Recombinant Proteins VP40 and NP

Проблемы особо опасных инфекций, 2010
Full-size genes coding matrix proteins VP40 of Ebola virus (VE) and Marburg virus (VM) and nucleoprotein (NP) of VE were cloned. These genes expression in Escherichia coli and purification of their products by affinity chromatography using Ni-NTA agarose
A. V. Ivanova, E. I. Kazachinskaya, A. V. Kachko, E. L. Subbotina, A. V. Sorokin, I. A. Razumov, S. V. Neteosov, V. B. Loktev   +7 more
doaj   +1 more source

Distinct Contributions of Different Domains within the HIV-1 Gag Polyprotein to Specific and Nonspecific Interactions with RNA

Viruses, 2020
Viral genomic RNA is packaged into virions with high specificity and selectivity. However, in vitro the Gag specificity towards viral RNA is obscured when measured in buffers containing physiological salt. Interestingly, when the binding is challenged by
Tomas Kroupa, Siddhartha A. K. Datta, Alan Rein   +2 more
doaj   +1 more source

Inhibition of the Protease Activity of Influenza Virus RNA Polymerase PA Subunit by Viral Matrix Protein [PDF]

Microbiology and Immunology, 2003
AbstractInfluenza virus PA is a subunit of RNA‐dependent RNA polymerase. We demonstrated that PA has a unique chymotrypsin‐like serine protease activity with Ser624 as an active site. To obtain further insight into the role of the protease activity of PA in viral proliferation, we examined the interaction between PA and matrix protein (M1).
Koyu Hara, Mayumi Shiota, Hiroshi Kido, Ken Watanabe, Kyosuke Nagata, Tetsuya Toyoda   +5 more
openalex   +3 more sources

Nucleotide sequence and genomic organization of an ophiovirus associated with lettuce big-vein disease [PDF]

, 2002
The complete nucleotide sequence of an ophiovirus associated with lettuce big-vein disease has been elucidated. The genome consisted of four RNA molecules of approximately 7ò8, 1ò7, 1ò5 and 1ò4 kb.
Dullemans, A.M., Heuvel, J.F.J.M., van den, Verbeek, M., Wilk, F., van der   +3 more
core   +2 more sources

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

Interaction of Host Nucleolin with Influenza A Virus Nucleoprotein in the Early Phase of Infection Limits the Late Viral Gene Expression. [PDF]

PLoS ONE, 2016
Influenza A virus nucleoprotein, is a multifunctional RNA-binding protein, encoded by segment-5 of the negative sense RNA genome. It serves as a key connector between the virus and the host during virus replication.
Deepshikha Kumar, Shobha Broor, Maitreyi S Rajala   +2 more
doaj   +1 more source