Results 111 to 120 of about 2,924,139 (391)

Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins

FEBS Open Bio, EarlyView.
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó, Eszter Nagy‐Kanta, Soma Varga, Edit Andrea Jáger, Csaba István Pongor, Mária Laki, András József Laki, Zoltán Gáspári   +7 more
wiley   +1 more source

Different evolutionary dynamics of hepatitis B virus genotypes A and D, and hepatitis D virus genotypes 1 and 2 in an endemic area of Yakutia, Russia

BMC Infectious Diseases, 2022
Background The geographic distribution of the hepatitis B virus (HBV) and the hepatitis D virus (HDV) genotypes is uneven. We reconstructed the temporal evolution of HBV and HDV in Yakutia, one of the regions of Russia most affected by HBV and HDV, in an
Anastasia A. Karlsen, Karen K. Kyuregyan, Olga V. Isaeva, Vera S. Kichatova, Fedor A. Asadi Mobarkhan, Lyudmila V. Bezuglova, Irina G. Netesova, Victor A. Manuylov, Andrey A. Pochtovyi, Vladimir A. Gushchin, Snezhana S. Sleptsova, Margarita E. Ignateva, Mikhail I. Mikhailov   +12 more
doaj   +1 more source

A brain microvasculature endothelial cell‐specific viral vector with the potential to treat neurovascular and neurological diseases

EMBO Molecular Medicine, 2016
Gene therapy critically relies on vectors that combine high transduction efficiency with a high degree of target specificity and that can be administered through a safe intravenous route. The lack of suitable vectors, especially for gene therapy of brain
Jakob Körbelin, Godwin K. Dogbevia, S. Michelfelder, D. Ridder, A. Hunger, Jan Wenzel, H. Seismann, Melanie Lampe, Jacqueline Bannach, M. Pasparakis, J. Kleinschmidt, M. Schwaninger, M. Trepel   +12 more
semanticscholar   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

RNA interference approaches for treatment of HIV-1 infection. [PDF]

, 2015
HIV/AIDS is a chronic and debilitating disease that cannot be cured with current antiretroviral drugs. While combinatorial antiretroviral therapy (cART) can potently suppress HIV-1 replication and delay the onset of AIDS, viral mutagenesis often leads to
Bobbin, Maggie L, Burnett, John C, Rossi, John J   +2 more
core   +1 more source

Replicating viral vector platform exploits alarmin signals for potent CD8+ T cell-mediated tumour immunotherapy

Nature Communications, 2017
Viral infections lead to alarmin release and elicit potent cytotoxic effector T lymphocyte (CTLeff) responses. Conversely, the induction of protective tumour-specific CTLeff and their recruitment into the tumour remain challenging tasks.
S. Kallert, Stephanie Darbre, Weldy V. Bonilla, M. Kreutzfeldt, Nicolas Page, P. Müller, M. Kreuzaler, Min Lu, S. Favre, F. Kreppel, M. Löhning, S. Luther, A. Zippelius, D. Merkler, D. Pinschewer   +14 more
semanticscholar   +1 more source

Viral and bacterial vectors of immunogenes

Vaccine, 1985
A recent development in the production of experimental vaccines has been the use of the smallpox vaccine virus (vaccinia virus) as a carrier (vector) of the genes (immunogenes) which code for the protection-inducing proteins (immunogens) of unrelated viruses.
openaire   +2 more sources

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Minimal hepatic toxicity of Onyx-015: spatial restriction of coxsackie-adenoviral receptor in normal liver. [PDF]

, 2007
We administered an adenoviral vector, Onyx-015, into the hepatic artery of patients with metastatic colorectal cancer involving the liver. Thirty-five patients enrolled in this multi-institutional phase I/II trial received up to eight arterial infusions ...
Au, T, Kirn, D, Korn, WM, Reid, TR, Sze, D, Thorne, S   +5 more
core  

Viral Vector-Based Targeting of miR-21 in Cardiac Nonmyocyte Cells Reduces Pathologic Remodeling of the Heart

Molecular Therapy, 2016
Systemic inhibition of miR-21 has proven effective against myocardial fibrosis and dysfunction, while studies in cardiac myocytes suggested a protective role in this cell type.
D. Ramanujam, Y. Sassi, B. Laggerbauer, S. Engelhardt   +3 more
semanticscholar   +1 more source