Results 161 to 170 of about 345,407 (349)

Cleanet: Robust Doublet Detection in Cytometry Data Based on Protein Expression Patterns

open access: yesCytometry Part A, EarlyView.
ABSTRACT Flow and mass cytometry experiments are essential for profiling immune cells at single‐cell resolution. Better understanding of human immunology increasingly involves analyzing studies at the scale of hundreds or thousands of samples, with data analysis a significant bottleneck.
Matei Ionita   +9 more
wiley   +1 more source

OMIP‐117: 40‐Parameter/37‐Color Spectral Cytometry Panel for Robust Immunoprofiling of Human Lymphoid Subsets in Cancer Patients

open access: yesCytometry Part A, EarlyView.
ABSTRACT The analysis of immune cell compartments in cancer patients is crucial to predict treatment efficacy and relapse. We introduce a robust 40‐parameter, 37‐channel spectral cytometry panel designed to profile human lymphoid subsets and CAR‐T cell expansion, with the capability to assess exhaustion status by profiling immune checkpoints and ...
Ondrej Venglar   +4 more
wiley   +1 more source

Monitoring Treatment‐Associated Receptor Expression in Multiple Sclerosis Using a Newly Developed Panel for Spectral Flow Cytometry

open access: yesCytometry Part A, EarlyView.
ABSTRACT Multiple sclerosis (MS) is a chronic, immune‐mediated autoimmune disease characterized by the infiltration of autoreactive T cells and other inflammatory immune cells from the periphery into the central nervous system. Currently, there is no cure for MS, and treatment consists of disease‐modifying therapies (DMTs), most of which modify or ...
Katharina Robichon   +4 more
wiley   +1 more source

OMIP‐118: A 38‐Marker Spectral Flow Cytometry Panel to Assess Human Regulatory T Cell Phenotype and Lineage Plasticity

open access: yesCytometry Part A, EarlyView.
ABSTRACT We report the optimization of a 38‐parameter spectral flow cytometry panel to identify, phenotype, and assess lineage plasticity of human regulatory T cells (Tregs). Tregs are an indispensable T cell lineage with pleiotropic tolerogenic functions whose activities contribute critically to numerous disease settings including cancer, autoimmunity,
Nilika Bhattacharya   +6 more
wiley   +1 more source

Validation of an Optimized DuraClone Phenotyping Kit Workflow for TBNK Subset Quantification

open access: yesCytometry Part A, EarlyView.
ABSTRACT Quantification of T‐cells, B‐cells, and NK‐cells assay is crucial for diagnosing and monitoring immune diseases and evaluating lymphodepleting therapies. To standardize and validate an optimized workflow of the Beckman Coulter DuraClone IM Phenotyping Basic Kit for quantification of TBNK subsets in peripheral blood. Procedural changes included
Carlos Agustin Villegas‐Valverde   +6 more
wiley   +1 more source

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