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Clade-specific multiplex digital PCR assay for monkeypox virus detection in wastewater
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Canadian Journal of Microbiology, 1977
Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyethylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water wash was noninfectious until treated with 0.5% polyethylene glycol.
J, Konowalchuk, J I, Spiers
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Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyethylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water wash was noninfectious until treated with 0.5% polyethylene glycol.
J, Konowalchuk, J I, Spiers
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Detection Methods for H1N1 Virus
2022Influenza A virus H1N1, a respiratory virus transmitted via droplets and responsible for the global pandemic in 2009, belongs to the Orthomyxoviridae family, a single-negative-stranded RNA. It possesses glycoprotein spikes neuraminidase (NA), hemagglutinin (HA), and a matrix protein named M2.
, Ravina +3 more
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IMMUNOELECTRONMICROSCOPY FOR VIRUS DETECTION AND VIRUS STRAIN IDENTIFICATION
Acta Horticulturae, 1983Potentialities of immunoelectronmicroscopy (IEM) for the detection of viruses have been clearly demonstrated. In particular, when used under optimal conditions, IEM appears as sensitive as ELISA. The best technique consists of the so-called immunosorbent electronmicroscopy (ISEM) followed by a coating of the trapped particles by specific antibodies ...
Kerlan, C., Mille, B., Dunez, Jean
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2010
Global incidence of dengue has increased considerably over the past decade. Dengue fever (DF) is a self-limiting disease; however, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are fatal. Since there is no therapy and vaccine against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried
Nagesh K, Tripathi +3 more
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Global incidence of dengue has increased considerably over the past decade. Dengue fever (DF) is a self-limiting disease; however, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are fatal. Since there is no therapy and vaccine against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried
Nagesh K, Tripathi +3 more
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Detection of avian encephalomyelitis virus
Research in Veterinary Science, 1986Methods for the detection of two strains of avian encephalomyelitis virus (AEV) in chick embryo brain cell cultures and chickens were compared. It was found that the agar gel precipitin test (AGPT) and the enzyme-linked immunosorbent assay (ELISA) carried out on the serum of inoculated chickens were more sensitive than either the indirect fluorescent ...
R A, Nicholas +3 more
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Detection of Zika virus in saliva
Journal of Clinical Virology, 2015During the largest Zika virus (ZIKV) outbreak ever reported that occurred from October 2013 to March 2014 in French Polynesia, we observed that several patients presenting the symptoms of acute phase Zika fever were tested negative in blood by ZIKV real-time PCR (RT-PCR).As we have previously detected ZIKV RNA in the saliva of a young child, we ...
Didier, Musso +5 more
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Detection and Prevention of Virus Infection
2022The pathogenic mechanism of viral infection is a complex process involving viral mutation, viral integration, and various aspects of the interaction between the viral genome and the host. Moreover, the virus mutation will lead to the failure of related vaccines, leading to the increasing of vaccine development costs and difficulties in virus prevention.
Ying, Wang, Bairong, Shen
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