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A Combination of M50I and V151I Polymorphic Mutations in HIV-1 Subtype B Integrase Results in Defects in Autoprocessing

open access: yesViruses, 2021
We have recently reported that a recombinant HIV-1NL4.3 containing Met-to-Ile change at codon 50 of integrase (IN) (IN:M50I) exhibits suppression of the virus release below 0.5% of WT HIV, and the released viral particles are replication-incompetent due ...
Jun Yang   +11 more
doaj   +1 more source

Isolation and Characterization of Two Pseudorabies Virus and Evaluation of Their Effects on Host Natural Immune Responses and Pathogenicity

open access: yesViruses, 2022
Pseudorabies, caused by the pseudorabies virus (PRV), is an acute fatal disease, which can infect rodents, mammals, and other livestock and wild animals across species.
Qiongqiong Zhou   +5 more
doaj   +1 more source

Transfection of RNA from organ samples of infected animals represents a highly sensitive method for virus detection and recovery of classical swine fever virus. [PDF]

open access: yesPLoS ONE, 2015
Translation and replication of positive stranded RNA viruses are directly initiated in the cellular cytoplasm after uncoating of the viral genome. Accordingly, infectious virus can be generated by transfection of RNA genomes into susceptible cells.
Denise Meyer   +3 more
doaj   +1 more source

Survey of mosquito species and mosquito-borne viruses in residential areas along the Sino–Vietnam border in Yunnan Province in China

open access: yesFrontiers in Microbiology, 2023
Mosquitoes are capable of carrying complex pathogens, and their feeding habits on the mammalian blood can easily mediate the spread of viruses. Surveillance of mosquito-based arbovirus enables the early prevention and control of mosquito-borne arboviral ...
Fengjuan Tian   +8 more
doaj   +1 more source

SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network [PDF]

open access: yesMemorias do Instituto Oswaldo Cruz, 2020
BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis ...
Danielle Bastos Araujo   +19 more
doaj   +1 more source

An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2020
Objective The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF.
Ha Thi Thanh Tran   +11 more
doaj   +1 more source

Respiratory Viral Shedding in Healthcare Workers Reinfected with SARS-CoV-2, Brazil, 2020

open access: yesEmerging Infectious Diseases, 2021
We documented 4 cases of severe acute respiratory syndrome coronavirus 2 reinfection by non–variant of concern strains among healthcare workers in Campinas, Brazil.
Mariene R. Amorim   +33 more
doaj   +1 more source

Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient

open access: yesEmerging Microbes and Infections, 2020
SARS-CoV-2 caused a major outbreak of severe pneumonia (COVID-19) in humans. Viral RNA was detected in multiple organs in COVID-19 patients. However, infectious SARS-CoV-2 was only isolated from respiratory specimens.
Jing Sun   +28 more
doaj   +1 more source

Isolation and identification of Circovirus in pigeon [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2021
The purpose of this study is first trial to detect of pigeon circovirus, so 1sr group include 100 cloacal swabs were collected 55 healthy and 45 ill pigeons, 36 yearlings and 64 adults, the 2nd group included organs was liver, spleen, bursa of Fabricius ...
Safwan Al-Baroodi, Mozahim Al-Attar
doaj   +1 more source

Virus diffusion in isolation rooms [PDF]

open access: yesJournal of Hospital Infection, 2006
In hospitals, the ventilation of isolation rooms operating under closed-door conditions is vital if the spread of viruses and infection is to be contained. Engineering simulation, which employs computational fluid dynamics, provides a convenient means of investigating airflow behaviour in isolation rooms for various ventilation arrangements.
Kao, P.H., Yang, R.J.
openaire   +2 more sources

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