Results 111 to 120 of about 1,696,709 (253)

Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome

Emerging Infectious Diseases, 2003
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS).
Rickjason C. W. Chan, John S. Tam, Ching-Wan Lam, Edward Chan, Alan Wu, Chi-Kong Li, Thomas A. Buckley, King-Cheung Ng, Gavin M. Joynt, Frankie W.T. Cheng, Ka-Fai To, Nelson Lee, David S.C. Hui, Jo L.K. Cheung, Ida Chu, Esther Liu, Sydney S.C. Chung, Joseph J.Y. Sung   +17 more
doaj   +1 more source

Waste Isolation Pilot Plant Environmental Monitoring Plan [PDF]

, 1999
DOE Order 5400.1, General Environmental Protection Program Requirements (DOE, 1990a), requires each DOE facility to prepare an EMP. This document is prepared for WIPP in accordance with the guidance contained in DOE Order 5400.1; DOE Order 5400.5 ...
Division, Westinghouse Electric Company Waste Isolation
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Epidemiological Investigation of Goose Astrovirus in Hebei Province, China, 2019–2021

Microorganisms
The goose astrovirus (GAstV), a key pathogen causing visceral gout and high mortality in geese, has spread widely in China, with frequent outbreaks in recent years.
Ligong Chen, Huan Cui, Jiaqi Li, Yuxin Zhang, Heng Wang, Yejin Yang, Xuejing Wang, Cheng Zhang, Juxiang Liu   +8 more
doaj   +1 more source

The Hepatitis C virus : your part in its downfall : getting to know the virus and its host [PDF]

, 2005
The cloning of the genome of the Hepatitis C virus (HCV) in 1989 provided sensitive screening tools and specific diagnostic assays which helped health workers to limit viral spread and scientists to design effective anti-viral agents.
Pullicino, Edgar
core  

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Genomic and phenotypic insights into the first imported monkeypox virus clade Ia isolate in China, 2025

Frontiers in Public Health
The 2022 multi-country outbreak of monkeypox virus (MPXV) sparked global health concerns, as cases emerged increasingly outside traditionally endemic regions. This outbreak, spanning 2022–2023, was primarily driven by the clade IIb strain.
Chunhong Yin, Chunhong Yin, Yan Li, Yan Li, Qing Duan, Qing Duan, Dali Xu, Dali Xu, Chengyunxiao Li, Ti Liu, Ti Liu, Ti Liu, Ti Liu, Shujun Ding, Shujun Ding, Zhe Zhang, Aihua Zhang, Lianchen Fu, Zengqiang Kou, Zengqiang Kou, Zengqiang Kou, Zengqiang Kou   +21 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source