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Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification [PDF]
Viruses, 2023 The quantification of viruses is necessary for both research and clinical applications. The methods available for RNA virus quantification possess several drawbacks, including sensitivity to inhibitors and the necessity of a standard curve generation ...
Ksenija Korotkaja, Anna Zajakina
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Quantification of Cytokine Storms During Virus Infections [PDF]
Frontiers in Immunology, 2021 Highly pathogenic virus infections usually trigger cytokine storms, which may have adverse effects on vital organs and result in high mortalities. The two cytokines interleukin (IL)-4 and interferon (IFN)-γ play key roles in the generation and regulation
Shu Yuan +5 more
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A Novel Transgenic Sf9 Cell Line for Quick and Easy Virus Quantification [PDF]
InsectsThe following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structures to
Kyu-Seek Kim +4 more
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Digital PCR as a tool to measure HIV persistence [PDF]
Retrovirology, 2018 Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Sofie Rutsaert +4 more
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Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples [PDF]
Diagnostics, 2023 Background: Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family Poxviridae. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and
Elena Pomari +6 more
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Quantification of Infectious Sendai Virus Using Plaque Assay
Bio-Protocol, 2018 Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy.
Narihito Tatsumoto +3 more
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Quantification of Ebola virus replication kinetics in vitro. [PDF]
PLoS Computational Biology, 2020 Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities for experimentation, modelling can play a crucial role.
Laura E Liao +15 more
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Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative Luciferase-Vpr packaging-based assay [PDF]
, 2011 The HIV-1 auxiliary protein Vpr and Vpr-fusion proteins can be copackaged with Gag precursor (Pr55Gag) into virions or membrane-enveloped virus-like particles (VLP).
Elisabeth Errazuriz +9 more
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Plants, 2023 Among the cucurbit-infecting viruses, watermelon mosaic virus (WMV) and zucchini yellow mosaic virus (ZYMV) (Potyvirus: Potyviridae) are responsible for severe symptoms on cucumber, melon, watermelon, and zucchini cultivations worldwide.
Marta Luigi +7 more
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Open Veterinary Journal, 2023 Background: Fowl adenovirus (FAdV) 8b and other serotypes cause inclusion body hepatitis (IBH) in chickens. Specific detection of aetiologic serotype in mixed infection and vaccine failure could be difficult.
Chidozie Clifford Ugwu +4 more
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