Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification [PDF]
Viruses, 2023 The quantification of viruses is necessary for both research and clinical applications. The methods available for RNA virus quantification possess several drawbacks, including sensitivity to inhibitors and the necessity of a standard curve generation ...
Ksenija Korotkaja, Anna Zajakina
doaj +3 more sources
A Novel Transgenic Sf9 Cell Line for Quick and Easy Virus Quantification [PDF]
InsectsThe following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structures to
Kyu-Seek Kim +4 more
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Quantification of Cytokine Storms During Virus Infections [PDF]
Frontiers in Immunology, 2021 Highly pathogenic virus infections usually trigger cytokine storms, which may have adverse effects on vital organs and result in high mortalities. The two cytokines interleukin (IL)-4 and interferon (IFN)-γ play key roles in the generation and regulation
Shu Yuan +5 more
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The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays. [PDF]
Biophys J, 2018 Many biological assays are employed in virology to quantify parameters of interest. Two such classes of assays, virus quantification assays (VQA) and infectivity assays (IA), aim to estimate the number of viruses present in a solution, and the ability of a viral strain to successfully infect a host cell, respectively.
Mistry BA, D'Orsogna MR, Chou T.
europepmc +10 more sources
Digital PCR as a tool to measure HIV persistence [PDF]
Retrovirology, 2018 Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Sofie Rutsaert +4 more
doaj +3 more sources
Standardization of Hepatitis C Virus RNA Quantification [PDF]
Hepatology, 2000 It was recently recommended that hepatitis C virus (HCV) RNA quantification be used to tailor the duration of combined interferon alfa (IFN-α)/ribavirin therapy in patients infected by HCV genotypes 1, 4, and 5. This recommendation has been difficult to implement in the absence of standardized quantitative units for HCV RNA ...
Jean‐Michel Pawlotsky +5 more
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Quantification of Ebola virus replication kinetics in vitro. [PDF]
PLoS Computational Biology, 2020 Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities for experimentation, modelling can play a crucial role.
Laura E Liao +15 more
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ddPCR for the Detection and Absolute Quantification of Oropouche Virus
VirusesBackground: Oropouche virus (OROV) is a segmented RNA virus belonging to the genus Orthobunyavirus in the family Peribunyaviridae. Herein, an in-house droplet digital PCR (ddPCR) assay was used for the detection and quantification of OROV.
Elena Pomari +6 more
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Quantification of Infectious Sendai Virus Using Plaque Assay
Bio-Protocol, 2018 Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy.
Narihito Tatsumoto +3 more
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In vivo imaging enables high resolution preclinical trials on patients' leukemia cells growing in mice. [PDF]
, 2012 Xenograft mouse models represent helpful tools for preclinical studies on human tumors. For modeling the complexity of the human disease, primary tumor cells are by far superior to established cell lines.
Brentjens, Renier J. +12 more
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