Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification [PDF]
Viruses, 2023 The quantification of viruses is necessary for both research and clinical applications. The methods available for RNA virus quantification possess several drawbacks, including sensitivity to inhibitors and the necessity of a standard curve generation ...
Ksenija Korotkaja, Anna Zajakina
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A Novel Transgenic Sf9 Cell Line for Quick and Easy Virus Quantification [PDF]
InsectsThe following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structures to
Kyu-Seek Kim +4 more
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Quantification of Cytokine Storms During Virus Infections [PDF]
Frontiers in Immunology, 2021 Highly pathogenic virus infections usually trigger cytokine storms, which may have adverse effects on vital organs and result in high mortalities. The two cytokines interleukin (IL)-4 and interferon (IFN)-γ play key roles in the generation and regulation
Shu Yuan +5 more
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The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays. [PDF]
Biophys J, 2018 Many biological assays are employed in virology to quantify parameters of interest. Two such classes of assays, virus quantification assays (VQA) and infectivity assays (IA), aim to estimate the number of viruses present in a solution, and the ability of
Mistry BA, D'Orsogna MR, Chou T.
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Clinical Application of Droplet Digital PCR for Hepatitis Delta Virus Quantification. [PDF]
Biomedicines, 2022 Droplet digital PCR (ddPCR) is a novel developed PCR technology providing the absolute quantification of target nucleic acid molecules without the need for a standard curve and regardless PCR amplification efficiency. Our aim was to develop a ddPCR assay
Olivero A +11 more
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Updating Sacbrood Virus Quantification PCR Method Using a TaqMan-MGB Probe. [PDF]
Vet Sci, 2021 Sacbrood virus (SBV) is a common honey bee virus disease. SBV variants and strains identified in Asian honey bees, Apis cerana, have created confusion in identifications.
Huang WF +5 more
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Digital Surface-Enhanced Raman Spectroscopy-Lateral Flow Test Dipstick: Ultrasensitive, Rapid Virus Quantification in Environmental Dust. [PDF]
Environ Sci TechnolThis study introduces a novel surface-enhanced Raman spectroscopy (SERS)-based lateral flow test (LFT) dipstick that integrates digital analysis for highly sensitive and rapid viral quantification.
Wang W +10 more
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Digital PCR as a tool to measure HIV persistence [PDF]
Retrovirology, 2018 Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Sofie Rutsaert +4 more
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Validation of Roche LightCycler Epstein-Barr Virus Quantification Reagents in a Clinical Laboratory Setting [PDF]
Journal of Molecular Diagnostics, 2006 Epstein-Barr virus (EBV) is associated with a wide range of benign and malignant diseases, including infectious mononucleosis, lymphoma, posttransplant lymphoproliferative disorder, and nasopharyngeal carcinoma. Measurement of EBV viral load in plasma is
Margaret L. Gulley +2 more
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Comparative Evaluation of Three Nucleic Acid-Based Assays for BK Virus Quantification. [PDF]
J Clin Microbiol, 2015 Descamps V +7 more
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