Results 101 to 110 of about 494,278 (351)

An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA

Memorias do Instituto Oswaldo Cruz, 2016
This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV ...
Ana Paula de Torres Santos, José Eduardo Levi, Marcilio Figueiredo Lemos, Samira Julien Calux, Isabel Takano Oba, Regina Célia Moreira   +5 more
doaj   +1 more source

Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]

, 2013
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki, Baldi, Beinke, Beinke, Beinke, Beinke, Beinke, Belich, Bouwmeester, Ciechanover, Cohen, Coope, Costa, Dauber, Dumitru, Durocher, Elke MÜHLBERGER, Feng, Gantke, Geisse, George, Gray, Jia, Jia, Julia Janzen, Kabouridis, Kaufman, Lang, Liu, Nicholas A. Morrice, Papoutsopoulou, Pomerantz, Robinson, Roget, Sabrina Boussouf, Salmeron, Salmeron, Savinova, Schlaeger, Schumann, Steven Howell, Steven C. Ley, Thorsten Gantke, Waterfield, Waterfield, Wu, Yang, Zhao   +47 more
core   +1 more source

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Quantification of Newly Synthesized Virus RNA in Moloney Murine Leukaemia Virus-infected Cells

Journal of General Virology, 1980
A technique for the isolation and characterization of newly transcribed murine leukaemia virus RNA in chronically infected cells has been developed. Cellular RNA was pulse labelled with 3H-uridine and virus-specific sequences were annealed with an excess of mercurated complementary DNA.
N, Guttman-Bass, H, Cedar, A, Panet
openaire   +2 more sources

Infrared laser sampling of low volumes combined with shotgun lipidomics reveals lipid markers in palatine tonsil carcinoma

Molecular Oncology, EarlyView.
Nanosecond infrared laser (NIRL) low‐volume sampling combined with shotgun lipidomics uncovers distinct lipidome alterations in oropharyngeal squamous cell carcinoma (OPSCC) of the palatine tonsil. Several lipid species consistently differentiate tumor from healthy tissue, highlighting their potential as diagnostic markers.
Leonard Kerkhoff, Manuela Moritz, Dennis Eggert, Anna Worthmann, Joerg Heeren, Henrike Zech, Till S. Clauditz, Waldemar Wilczak, Hartmut Schlüter, Christian S. Betz, Arne Böttcher, Jan Hahn   +11 more
wiley   +1 more source

Switching from a protease inhibitor-based regimen to a dolutegravir-based regimen : a randomized clinical trial to determine the effect on peripheral blood and ileum biopsies from antiretroviral therapy-suppressed human immunodeficiency virus-infected individuals [PDF]

, 2018
Background: Optimization of combination antiretroviral therapy (cART) can impact the human immunodeficiency virus (HIV) reservoir. We evaluated the effect on the HIV reservoir in peripheral blood and ileum biopsies in patients switching from boosted ...
Blanco, Julià, Crespo, Manel, De Clercq, Laura, Gálvez, Cristina, Jimenez, Montse, Martinez-Picado, Javier, Moron-Lopez, Sara, Navarro, Jordi, Puertas, Maria C, Ribas, Bibiana Planas, Rutsaert, Sofie, Salgado, Maria, Torrella, Ariadna, Universitat Autònoma de Barcelona, Urrea, Víctor, Vandekerckhove, Linos   +15 more
core   +3 more sources

Recurrent cancer‐associated ERBB4 mutations are transforming and confer resistance to targeted therapies

Molecular Oncology, EarlyView.
We show that the majority of the 18 analyzed recurrent cancer‐associated ERBB4 mutations are transforming. The most potent mutations are activating, co‐operate with other ERBB receptors, and are sensitive to pan‐ERBB inhibitors. Activating ERBB4 mutations also promote therapy resistance in EGFR‐mutant lung cancer.
Veera K. Ojala, Sini Ahonen, Sara Peltola, Aura Tuohisto‐Kokko, Olaya Esparta, Peppi Suominen, Anne Jokilammi, Iman Farahani, Deepankar Chakroborty, Nikol Dibus, Steffen Boettcher, Tomi T. Airenne, Mark S. Johnson, Lisa D. Eli, Klaus Elenius, Kari J. Kurppa   +15 more
wiley   +1 more source

Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China

Journal of Veterinary Research, 2016
Introduction: A real-time RT-PCR method for identification and quantification of porcine epidemic diarrhoea virus (PEDV) strains in China was developed.
Hou Yi-Xuan, Xie Chun, Wang Kang, Zhao Yu-Ting, Xie Yang-Yang, Shi Hong-Yan, Chen Jian-Fei, Feng Li, Tong Guang-Zhi, Hua Xiu-Guo, Yuan Cong-Li, Zhou Yan-Jun, Yang Zhi-Biao   +12 more
doaj   +1 more source

One-Step Reverse-Transcription Digital PCR for Reliable Quantification of Different Pepino Mosaic Virus Genotypes

Plants, 2020
In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide.
Nataša Mehle, Larisa Gregur, Alexandra Bogožalec Košir, David Dobnik   +3 more
doaj   +1 more source

Syndromic surveillance and pathogen detection using multiplex assays for respiratory infections in small ruminants [PDF]

, 2018
Background Several bacterial and viruses can infect the respiratory tract of small ruminants causing similar clinical signs. The differential diagnosis of respiratory diseases in small ruminants can be achieved using multi-plex assays for an accurate ...
Cattoli, Giovanni, Diallo, Adama, Euloge Lamien, Charles, Loitsch, Angelika, Settypalli, Tirumala Bharani Kumar, Tounkara, Kadidia   +5 more
core