Results 121 to 130 of about 3,645,587 (361)

Immune- and nonimmune-compartment-specific interferon responses are critical determinants of herpes simplex virus-induced generalized infections and acute liver failure [PDF]

, 2016
The interferon (IFN) response to viral pathogens is critical for host survival. In humans and mouse models, defects in IFN responses can result in lethal herpes simplex virus 1 (HSV-1) infections, usually from encephalitis.
Leib, David A, Parker, George A, Parker, Zachary M, Pasieka, Tracy Jo   +3 more
core   +3 more sources

Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss

FEBS Open Bio, EarlyView.
Mitochondria contain two mitoribosome rescue factors, ICT1 and MTRFR (C12orf65). ICT1 also functions as a mitoribosomal protein in mice and humans, and its loss is lethal. Although Mtrfr knockout mice could not be generated, knockout zebrafish lines for ict1 and mtrfr were established.
Nobukazu Nameki, Chika Tomisawa, Soichiro Hoshino, Hidehiko Shimizu, Masashi Abe, Sho Arai, Kanako Kuwasako, Naoki Asakawa, Yusuke Inoue, Takuro Horii, Izuho Hatada, Masakatsu Watanabe   +11 more
wiley   +1 more source

Effector memory differentiation increases detection of replication-competent HIV-l in resting CD4+ T cells from virally suppressed individuals. [PDF]

, 2019
Studies have demonstrated that intensive ART alone is not capable of eradicating HIV-1, as the virus rebounds within a few weeks upon treatment interruption.
Bale, Michael J, Busch, Michael P, Cox, Bryan, Deeks, Steven G, Hoh, Rebecca, Kearney, Mary F, Kulpa, Deanna A, Lackman-Smith, Carol, Maldarelli, Frank, Ptak, Roger G, Stone, Mars, Subramanian, Krupa, Wiegand, Ann, Wonderlich, Elizabeth R   +13 more
core   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

A flow cytometric granularity assay for the quantification of infectious virus

Vaccine, 2019
A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples.
Megan Logan, Julia Manalil, Christina Notte, Craig Kearse, Steve George, Arno Zeiser, Patrick Farrell, Marc G. Aucoin   +7 more
openaire   +3 more sources

Quantification of Lyssavirus-Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudotype Particles

Viruses, 2016
Rabies is a highly fatal zoonotic disease which is primarily caused by rabies virus (RABV) although other members of the genus Lyssavirus can cause rabies as well. As yet, 14 serologically and genetically diverse lyssaviruses have been identified, mostly
Sarah Moeschler, Samira Locher, K. Conzelmann, B. Krämer, G. Zimmer   +4 more
semanticscholar   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

West Nile virus quantification in feces of experimentally infected American and fish crows.

American Journal of Tropical Medicine and Hygiene, 2006
To better understand the potential environmental health risk presented by West Nile virus (WNV)-contaminated feces, we quantified the amount of WNV present in the feces of experimentally infected American crows (Corvus brachyrhynchos) and fish crows ...
A. Kipp, J. Lehman, R. Bowen, P. Fox, Michael R Stephens, Kaci Klenk, N. Komar, M. Bunning   +7 more
semanticscholar   +1 more source

Technical development and In Silico implementation of SyntheticMR in head and neck adaptive radiation therapy: A prospective R‐IDEAL stage 0/1 technology development report

Journal of Applied Clinical Medical Physics, EarlyView.
Abstract Background SyntheticMR has the capability of generating quantitative relaxometry maps and synthetic contrast‐weighted MRI images in rapid acquisition times. Recently, it has gained attention in the diagnostic community, however, no studies have investigated its feasibility on the MR‐Simulation or MR‐Linac systems, especially as part of the ...
Lucas McCullum, Samuel L. Mulder, Natalie A. West, Robert Aghoghovbia, Alaa Mohamed Shawky Ali, Hayden Scott, Travis C. Salzillo, Yao Ding, Alex Dresner, Ergys Subashi, Dan Ma, R. Jason Stafford, Ken‐Pin Hwang, Clifton D. Fuller   +13 more
wiley   +1 more source

Duplex Droplet Digital PCR Assay for Quantification of Hepatitis E Virus in Food

Viruses
Hepatitis E virus (HEV) represents an emerging risk in industrialized countries where the consumption of contaminated food plays a pivotal role.
Gianfranco La Bella, Maria Grazia Basanisi, Gaia Nobili, Anna Mattea D’Antuono, Elisabetta Suffredini, Giovanna La Salandra   +5 more
doaj   +1 more source