Results 11 to 20 of about 494,278 (351)

Evaluating Novel Quantification Methods for Infectious Baculoviruses

open access: yesViruses, 2023
Accurate and rapid quantification of (infectious) virus titers is of paramount importance in the manufacture of viral vectors and vaccines. Reliable quantification data allow efficient process development at a laboratory scale and thorough process ...
Keven Lothert   +2 more
doaj   +1 more source

Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens

open access: yesDiagnostics, 2021
Detection and quantification of viruses in laboratory and clinical samples are standard assays in dengue virus (DENV) studies. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) is considered to be the standard for DENV detection ...
Dumrong Mairiang   +17 more
doaj   +1 more source

The Interaction between B87 Vaccine Strain and BC6/85 of Infectious Bursa Disease Virus in SPF Chickens

open access: yesViruses, 2022
This study was initiated to determine the interaction between two infectious bursal disease virus (IBDV) strains in the early stages of infection by detection and quantification of IBDV RNA in lymphoid and non-lymphoid tissues.
Ling Chen   +3 more
doaj   +1 more source

Development and Preliminary Application of a Droplet Digital PCR Assay for Quantifying the Oncolytic Herpes Simplex Virus Type 1 in the Clinical-Grade Production

open access: yesViruses, 2023
Oncolytic herpes simplex virus (oHSV) is a type of virus that selectively targets and kills cancer cells, leaving normal cells unharmed. Accurate viral titer is of great importance for the production and application of oHSV products. Droplet digital PCR (
Miaomiao Guo   +10 more
doaj   +1 more source

Concentration and quantification of Tilapia tilapinevirus from water using a simple iron flocculation coupled with probe-based RT-qPCR [PDF]

open access: yesPeerJ, 2022
Background Tilapia tilapinevirus, also known as tilapia lake virus (TiLV), is a significant virus that is responsible for the die-off of farmed tilapia across the globe.
Suwimon Taengphu   +6 more
doaj   +2 more sources

Virus detection and quantification using electrical parameters [PDF]

open access: yesScientific Reports, 2014
Here we identify and quantitate two similar viruses, human and feline immunodeficiency viruses (HIV and FIV), suspended in a liquid medium without labeling, using a semiconductor technique. The virus count was estimated by calculating the impurities inside a defined volume by observing the change in electrical parameters.
Mahmoud Al Ahmad   +3 more
openaire   +2 more sources

Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

open access: yesViruses, 2022
Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify the host factors involved and treatments to combat infection. Quantification of released virions often requires lengthy procedures, whereas quantification of viral RNA in ...
Nicky Craig   +7 more
doaj   +1 more source

Zika Virus: Quantification, Propagation, Detection, and Storage [PDF]

open access: yesCurrent Protocols in Microbiology, 2016
AbstractZika virus (ZIKV), belonging to the family Flaviviridae, genus Flavivirus, is an arthropod‐borne virus that was first discovered from the Zika forest in Uganda in 1947. Recent outbreaks in South America have linked ZIKV to cases of microcephaly and Guillain‐Barré syndrome in humans.
Darrell S, Agbulos   +3 more
openaire   +2 more sources

Honey Bee Queens and Virus Infections

open access: yesViruses, 2020
The honey bee queen is the central hub of a colony to produce eggs and release pheromones to maintain social cohesion. Among many environmental stresses, viruses are a major concern to compromise the queen’s health and reproductive vigor.
Esmaeil Amiri   +3 more
doaj   +1 more source

Influenza A virus encoding secreted Gaussia luciferase as useful tool to analyze viral replication and its inhibition by antiviral compounds and cellular proteins. [PDF]

open access: yesPLoS ONE, 2014
Reporter genes inserted into viral genomes enable the easy and rapid quantification of virus replication, which is instrumental to efficient in vitro screening of antiviral compounds or in vivo analysis of viral spread and pathogenesis.
Nadine Eckert   +7 more
doaj   +1 more source

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