Results 11 to 20 of about 3,671,396 (364)

Zika Virus: Quantification, Propagation, Detection, and Storage [PDF]

Current Protocols in Microbiology, 2016
AbstractZika virus (ZIKV), belonging to the family Flaviviridae, genus Flavivirus, is an arthropod‐borne virus that was first discovered from the Zika forest in Uganda in 1947. Recent outbreaks in South America have linked ZIKV to cases of microcephaly and Guillain‐Barré syndrome in humans.
Fiona F. Hunter, Darrell S. Agbulos, Larissa Barelli, Bryan V. Giordano   +3 more
openaire   +4 more sources

The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus

Current Protocols in Microbiology, 2020
AbstractVesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative‐sense single‐stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also
Alaa A. Abdelmageed, Maureen C. Ferran
openaire   +4 more sources

Clinical Application of Droplet Digital PCR for Hepatitis Delta Virus Quantification. [PDF]

Biomedicines, 2022
Droplet digital PCR (ddPCR) is a novel developed PCR technology providing the absolute quantification of target nucleic acid molecules without the need for a standard curve and regardless PCR amplification efficiency. Our aim was to develop a ddPCR assay
Olivero A, Rosso C, Ciancio A, Abate ML, Nicolosi A, Troshina G, Armandi A, Ribaldone DG, Saracco GM, Bugianesi E, Rizzetto M, Caviglia GP.   +11 more
europepmc   +2 more sources

Updating Sacbrood Virus Quantification PCR Method Using a TaqMan-MGB Probe. [PDF]

Vet Sci, 2021
Sacbrood virus (SBV) is a common honey bee virus disease. SBV variants and strains identified in Asian honey bees, Apis cerana, have created confusion in identifications.
Huang WF, Zhang Y, Mehmood S, Wang Z, Hou C, Li Z.   +5 more
europepmc   +2 more sources

Quantification of Influenza Virus RNA in Aerosols in Patient Rooms

PLOS ONE, 2016
The potential for human influenza viruses to spread through fine particle aerosols remains controversial. The objective of our study was to determine whether influenza viruses could be detected in fine particles in hospital rooms.We sampled the air in 2-bed patient isolation rooms for four hours, placing cyclone samplers at heights of 1.5m and 1.0m. We
Chu, KW, Beezhold, DH, Li, Y, Yen, H, Seto, WH, Peiris, JSM, Cowling, BJ, Lindsley, WG, Yu, H, Leung, NHL, Zhou, J   +10 more
openaire   +8 more sources

Digital Surface-Enhanced Raman Spectroscopy-Lateral Flow Test Dipstick: Ultrasensitive, Rapid Virus Quantification in Environmental Dust. [PDF]

Environ Sci Technol
This study introduces a novel surface-enhanced Raman spectroscopy (SERS)-based lateral flow test (LFT) dipstick that integrates digital analysis for highly sensitive and rapid viral quantification.
Wang W, Srivastava S, Garg A, Xiao C, Hawks S, Pan J, Duggal N, Isaacman-VanWertz G, Zhou W, Marr LC, Vikesland PJ.   +10 more
europepmc   +2 more sources

Development, Validation, and Application of Reverse Transcription Real-Time and Droplet Digital PCR Assays for the Detection of the Potyviruses Watermelon Mosaic Virus and Zucchini Yellow Mosaic Virus in Cucurbits

Plants, 2023
Among the cucurbit-infecting viruses, watermelon mosaic virus (WMV) and zucchini yellow mosaic virus (ZYMV) (Potyvirus: Potyviridae) are responsible for severe symptoms on cucumber, melon, watermelon, and zucchini cultivations worldwide.
Marta Luigi, Ariana Manglli, Carla Libia Corrado, Antonio Tiberini, Elisa Costantini, Luca Ferretti, Laura Tomassoli, Sabrina Bertin   +7 more
doaj   +1 more source

Comparative Evaluation of Three Nucleic Acid-Based Assays for BK Virus Quantification. [PDF]

J Clin Microbiol, 2015
Descamps V, Martin E, Morel V, François C, Helle F, Duverlie G, Castelain S, Brochot E.   +7 more
europepmc   +2 more sources

TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus

Open Veterinary Journal, 2023
Background: Fowl adenovirus (FAdV) 8b and other serotypes cause inclusion body hepatitis (IBH) in chickens. Specific detection of aetiologic serotype in mixed infection and vaccine failure could be difficult.
Chidozie Clifford Ugwu, Mohd Hair Bejo, Abdul Rahman Omar, Nurulfiza Mat Isa, Aini Ideris   +4 more
doaj   +1 more source

Virus Quantification Methods with Focus on Marek's Disease Viruses

Journal of Poultry Sciences and Avian Diseases, 2023
Marek's disease (MD) is a significant concern in the poultry industry, causing neoplastic disease and substantial economic losses. MDV-1, MDV-2, and MDV-3 are the three serotypes of the herpesvirus that cause MD, each with unique traits and interactions ...
Mohammad Reza Piriaei, J. Razmyar, Sara Dolatyabi, M. H. Bozorgmehrifard   +3 more
semanticscholar   +1 more source