Results 311 to 320 of about 494,278 (351)

Sindbis Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2005
AbstractThe prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a complex transmission cycle. Sindbis virus must, therefore, be able to replicate in two biochemically and genetically divergent hosts, invertebrates and ...
Raquel, Hernandez, Christine, Sinodis, Dennis T, Brown   +2 more
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Dengue Virus: Isolation, Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2012
AbstractDengue is a disease caused by infection with one of the four dengue virus serotypes (DENV‐1, ‐2, ‐3, and ‐4). The virus is transmitted to humans by Aedes sp. mosquitoes. This enveloped virus contains a positive single‐stranded RNA genome. Clinical manifestations of dengue can have a wide range of outcomes varying from a mild febrile illness to ...
Freddy, Medina, Juan Francisco, Medina, Candimar, Colón, Edgardo, Vergne, Gilberto A, Santiago, Jorge L, Muñoz-Jordán   +5 more
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Review of Detection and Quantification of Rabies Virus Antibodies

Viral Immunology, 2021
Rabies is an almost invariably fatal disease. According to the World Health Organization (WHO), rabies virus neutralizing antibody (RVNA) titers of ≥0.5 IU/mL are considered adequate for rabies protection. Therefore, detection and quantification of RABV antibodies are important. Many methods have been developed for detecting RABV antibodies.
Kewen Su, Jian Xue, Xiaoyue Shan, Haipeng Ye, Ling Zhang, Siwei Tan, Ji Shao, Yanpeng Shi, Zhe Wang, Lei Zhang   +9 more
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Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay

2016
The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via
Parveen, Kaur, Regina Ching Hua, Lee, Justin Jang Hann, Chu   +2 more
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Herpes Simplex Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2006
AbstractHerpes simplex virus (HSV) is the prototype of a family of large, enveloped, double‐stranded DNA viruses, the Herpesviridae, which cause significant morbidity and mortality in humans. Productive replication of HSV in cells in culture results in definitive changes in cellular physiology and metabolism, ultimately leading to lysis.
John A, Blaho, Elise R, Morton, Jamie C, Yedowitz   +2 more
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Newcastle Disease Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2006
AbstractNewcastle disease virus (NDV) is a prototype paramyxovirus used to define basic steps in the life cycle of this family of viruses. NDV is also an ideal virus system for elucidating determinants of viral pathogenicity. Some strains of this virus are important agricultural pathogens that cause disease in poultry with a high mortality while other ...
Lori W, McGinnes, Homer, Pantua, Julie, Reitter, Trudy G, Morrison   +3 more
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Quantification of West Nile Virus by Plaque-Forming Assay

2022
The plaque-forming assay is a gold standard technique to determine the concentration of infectious viral particles. In this assay, lytic viruses infect and lyse the cells but are immobilized due to the presence of an agarose-containing overlay medium.
Neupane, Biswas, Bai, Fengwei
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Experimental quantification of vaccine-induced reduction in virus transmission

Vaccine, 1994
Although reduction in transmission of an agent in the host population is an important goal of many vaccinations, suitable experimental methods to measure transmission have been lacking. Therefore, we designed and tested an animal experiment to quantify transmission among vaccinated and unvaccinated animals.
M C, De Jong, T G, Kimman
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Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification

Current Protocols in Microbiology, 2018
AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is a member of the familyArteriviridae, orderNidovirale. PRRSV is an enveloped, single‐stranded, positive‐sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC‐145 cell line is the most often used in a laboratory setting.
Zexu, Ma, Liping, Yang, Yan-Jin, Zhang
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Bluetongue Virus (BTV): Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2012
AbstractAs an obligate intracellular parasite, the genome of the Bluetongue virus (BTV) contains ten double‐stranded RNA segments which are encapsidated by viral proteins, forming “transport vesicles” that can transmit the viral progeny cell‐to‐cell efficiently and that can also be transmitted animal‐to‐animal by a biting midge.
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