Results 31 to 40 of about 494,278 (351)

Hepatitis E Virus: Isolation, Propagation, and Quantification

Current Protocols, 2018
AbstractHepatitis E virus (HEV) predominantly causes acute liver disease in humans and is transmitted via the fecal‐oral route. HEV infection in pregnant women can result in grave consequences, with up to 30% fatality. The HEV strains infecting humans mainly belong to four genotypes.
Lin, Shaoli, Yang, Liping, Zhang, Yan-Jin   +2 more
openaire   +4 more sources

Quantification of virus-infected cells using RNA FISH-Flow

STAR Protocols, 2023
We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA. RNA FISH-Flow probes can be synthesized to match any RNA virus genome, in either sense or anti-sense, enabling ...
Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer   +6 more
openaire   +3 more sources

One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes

Engineered Regeneration, 2023
Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored.
Dagan Zhang, Yuze Wang, Junqi Zhao, Xueqin Li, Yuanyang Zhou, Sen Wang   +5 more
doaj   +1 more source

Cellular IP₆ Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP₆ Are Attenuated for Infection and Replication [PDF]

, 2019
Summary: HIV-1 hijacks host proteins to promote infection. Here we show that HIV is also dependent upon the host metabolite inositol hexakisphosphate (IP6) for viral production and primary cell replication.
Böcking, Till, Faysal, K. M.Rifat, Fletcher, Adam J., Freed, Eric O., James, Leo C., Kleinpeter, Alex, Mallery, Donna L., Novikova, Mariia, Saiardi, Adolfo, Vaysburd, Marina, Wilson, Miranda S.C.   +10 more
core   +3 more sources

Zika virus isolation, propagation, and quantification using multiple methods

PLOS ONE, 2021
Zika virus (ZIKV) was isolated from the archival urine, serum, and autopsy specimens by intrathoracic inoculation of Toxorhynchitis splendens and followed by three blind sub-passaging in C6/36 mosquito cells. The virus isolates were identified using an immunofluorescence assay and real-time reverse transcription-polymerase chain reaction (real-time RT ...
Worawat Dangsagul, Kriengsak Ruchusatsawat, Apiwat Tawatsin, Don Changsom, Pirom Noisumdaeng, Sukontip Putchakarn, Chayawat Phatihattakorn, Prasert Auewarakul, Pilaipan Puthavathana   +8 more
openaire   +4 more sources

Effector memory differentiation increases detection of replication-competent HIV-l in resting CD4+ T cells from virally suppressed individuals. [PDF]

, 2019
Studies have demonstrated that intensive ART alone is not capable of eradicating HIV-1, as the virus rebounds within a few weeks upon treatment interruption.
Bale, Michael J, Busch, Michael P, Cox, Bryan, Deeks, Steven G, Hoh, Rebecca, Kearney, Mary F, Kulpa, Deanna A, Lackman-Smith, Carol, Maldarelli, Frank, Ptak, Roger G, Stone, Mars, Subramanian, Krupa, Wiegand, Ann, Wonderlich, Elizabeth R   +13 more
core   +1 more source

Immunoplaque Assay (Influenza Virus)

Bio-Protocol, 2013
Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the assay ...
Victor Tse, Yueting Zhang, Gary Whittaker   +2 more
doaj   +1 more source

Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye [PDF]

, 2016
BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of ...
Ausimmune Investigator Group (AIG), Dwyer, Dominic E, Lay, Meav-Lang J, Lucas, Robyn, Ponsonby, Anne-Louise, Ratnamohan, Mala, Taylor, Janette   +6 more
core   +1 more source

Hepatitis C Virus: Propagation, Quantification, and Storage [PDF]

Current Protocols in Microbiology, 2010
AbstractHepatitis C virus (HCV) is a leading cause of chronic liver diseases, including chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV is well known for its restricted tropism and it does not replicate well in animal species other than humans and chimpanzees. Since classical in vitro propagation of natural HCV isolates is not possible,
openaire   +2 more sources

Deep Transfer Learning Approach for Automatic Recognition of Drug Toxicity and Inhibition of SARS-CoV-2

Viruses, 2021
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes COVID-19 and is responsible for the ongoing pandemic. Screening of potential antiviral drugs against SARS-CoV-2 depend on in vitro experiments, which are based on the quantification of ...
Julia Werner, Raphael M. Kronberg, Pawel Stachura, Philipp N. Ostermann, Lisa Müller, Heiner Schaal, Sanil Bhatia, Jakob N. Kather, Arndt Borkhardt, Aleksandra A. Pandyra, Karl S. Lang, Philipp A. Lang   +11 more
doaj   +1 more source