Results 31 to 40 of about 3,645,587 (361)

Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

Viruses, 2022
Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify the host factors involved and treatments to combat infection. Quantification of released virions often requires lengthy procedures, whereas quantification of viral RNA in ...
Nicky Craig, Sarah L. Fletcher, Alison Daniels, Caitlin Newman, Marie O’Shea, Wenfang Spring Tan, Amanda Warr, Christine Tait-Burkard   +7 more
doaj   +1 more source

Honey Bee Queens and Virus Infections

Viruses, 2020
The honey bee queen is the central hub of a colony to produce eggs and release pheromones to maintain social cohesion. Among many environmental stresses, viruses are a major concern to compromise the queen’s health and reproductive vigor.
Esmaeil Amiri, Micheline K. Strand, David R. Tarpy, Olav Rueppell   +3 more
doaj   +1 more source

Influenza A virus encoding secreted Gaussia luciferase as useful tool to analyze viral replication and its inhibition by antiviral compounds and cellular proteins. [PDF]

PLoS ONE, 2014
Reporter genes inserted into viral genomes enable the easy and rapid quantification of virus replication, which is instrumental to efficient in vitro screening of antiviral compounds or in vivo analysis of viral spread and pathogenesis.
Nadine Eckert, Florian Wrensch, Sabine Gärtner, Navaneethan Palanisamy, Ulrike Goedecke, Nils Jäger, Stefan Pöhlmann, Michael Winkler   +7 more
doaj   +1 more source

Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples

Diagnostics, 2023
Background: Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family Poxviridae. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and
Elena Pomari, Antonio Mori, Silvia Accordini, Annalisa Donini, Maddalena Cordioli, Evelina Tacconelli, Concetta Castilletti   +6 more
doaj   +1 more source

Applicability of Different Methods for Quantifying Virucidal Efficacy Using MENNO Florades and Tomato Brown Rugose Fruit Virus as an Example

Plants, 2023
After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) shall be disinfected. Therefore, the selection of an effective disinfectant plays an important role.
Shaheen Nourinejhad Zarghani, Jens Ehlers, Mehran Monavari, Susanne von Bargen, Joachim Hamacher, Carmen Büttner, Martina Bandte   +6 more
doaj   +1 more source

Vaccination reduces the viral load and the risk of transmission of Jembrana disease virus in Bali cattle [PDF]

, 2009
The efficacy of a tissue-derived vaccine, which is currently used in Indonesia to control the spread of Jembrana disease in Bali cattle, was determined by quantifying the viral load in plasma following experimental infection with Jembrana disease virus ...
Desport, M., Ditcham, W.G.F., Dobson, R.J., Hartaningsih, N., Lewis, J.R., Wilcox, G.E.   +5 more
core   +2 more sources

Quantification of Influenza Virus Messenger RNAs

Journal of General Virology, 1980
The amounts of specific influenza virus cRNAs present on polyribosomes of cells treated with cycloheximide at various times after infection were measured by quantitative hybridization with 125I-labelled virus RNA segments. All species of cRNA were found associated with polyribosomes at every time point analysed.
Patricia A. Tekamp, Edward E. Penhoet
openaire   +3 more sources

Quantifying Lipid Contents in Enveloped Virus Particles with Plasmonic Nanoparticles [PDF]

, 2014
Phosphatidylserine (PS) and monosialotetrahexosylganglioside (GM1) are examples of two host-derived lipids in the membrane of enveloped virus particles that are known to contribute to virus attachment, uptake, and ultimately dissemination. A quantitative
Aaron, Akiyama, Bavari, Brugger, Brugger, Callahan, Chan, Cooling, Crow, Dreaden, Elghanian, Encina, Epand, Facke, Fiebig, Fujita, Grassme, Grgacic, Halas, Han, Haslam, Hatch, Hu, Hu, Izquierdo-Useros, Jain, Jain, Jemielity, Johnson, Joshi, Jun, Kawasaki, Kelly, Koivusalo, Kuziemko, Lehmann, Leung, Leventis, Makino, Mercer, Moller-Tank, Nehl, Nordlander, Ono, Puryear, Puryear, Puryear, Rechberger, Reinhard, Reinhard, Schiralli Lester, Skewis, Soares, Sonnichsen, Su, Suzuki, Tsai, van, Wang, Wang, Wang, Willets, Wu, Yan, Yan, Yan, Yang, Yguerabide, Yguerabide, Yu, Yu, Zhang, Zhang   +72 more
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Quantitative detection of _Potato virus Y_ in potato plants and aphids - Discussion of diverse applications in potato research [PDF]

, 2011
Every year potato growers worldwide complain about severe yield losses caused by _Potato virus Y_ (PVY). Therefore, PVY along with _Potato leafroll virus_ belongs to the most important potato viruses.
Anja Hü, Edgar Schliephake, Jö, Thomas Thieme   +3 more
core   +2 more sources

The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility [PDF]

, 2013
Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs).
Alberts, Alvarez, Arakawa, Bagrodia, Bernardini, Block, Boujemaa-Paterski, Chong, Chong, Diego E. Alvarez, Earl, Engelstad, Feig, Frischknecht, Fukuoka, Gasteier, Goldberg, Haglund, Hannemann, Harris, Harris, Heindl, Hervé Agaisse, Hiller, Hollinshead, Hoppe, Humphries, Isaacs, Katz, Kleba, Koka, Kovar, Krall, Lammers, Lasa, Li, Madrid, Moseley, Moseley, Newsome, Newsome, Nezami, Otomo, Parkinson, Pollard, Pring, Pruyne, Qiu, Ridley, Rietdorf, Roberts, Rolls, Romero, Röttger, Sanderson, Scaplehorn, Schmelz, Schulte, Schönichen, Schönichen, Serio, Shibata, Siripala, Skoble, Smith, Suzuki, Takeya, Tooze, van Eijl, van Eijl, Wallar, Ward, Ward, Watanabe, Weisswange, Welch, Westendorf, Westendorf, Wolffe, Wolffe   +79 more
core   +2 more sources