Honey Bee Queens and Virus Infections
The honey bee queen is the central hub of a colony to produce eggs and release pheromones to maintain social cohesion. Among many environmental stresses, viruses are a major concern to compromise the queen’s health and reproductive vigor.
Esmaeil Amiri+3 more
doaj +1 more source
Background: Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family Poxviridae. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and
Elena Pomari+6 more
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Influenza A virus encoding secreted Gaussia luciferase as useful tool to analyze viral replication and its inhibition by antiviral compounds and cellular proteins. [PDF]
Reporter genes inserted into viral genomes enable the easy and rapid quantification of virus replication, which is instrumental to efficient in vitro screening of antiviral compounds or in vivo analysis of viral spread and pathogenesis.
Nadine Eckert+7 more
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After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) shall be disinfected. Therefore, the selection of an effective disinfectant plays an important role.
Shaheen Nourinejhad Zarghani+6 more
doaj +1 more source
Methods for virus recovery from environmental surfaces to monitor infectious viral contamination
Accurate quantification of infectious contaminants on environmental surfaces, particularly infectious viruses, is essential for contact transmission risk assessment; however, difficulties in recovering viruses from surfaces using swabs complicates this ...
Hajime Miyazaki+6 more
doaj +1 more source
Quantifying Lipid Contents in Enveloped Virus Particles with Plasmonic Nanoparticles [PDF]
Phosphatidylserine (PS) and monosialotetrahexosylganglioside (GM1) are examples of two host-derived lipids in the membrane of enveloped virus particles that are known to contribute to virus attachment, uptake, and ultimately dissemination. A quantitative
Aaron+72 more
core +2 more sources
Quantification of Influenza Virus Messenger RNAs
The amounts of specific influenza virus cRNAs present on polyribosomes of cells treated with cycloheximide at various times after infection were measured by quantitative hybridization with 125I-labelled virus RNA segments. All species of cRNA were found associated with polyribosomes at every time point analysed.
Patricia A. Tekamp, Edward E. Penhoet
openaire +3 more sources
Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye [PDF]
BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of ...
Ausimmune Investigator Group (AIG)+6 more
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One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes
Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored.
Dagan Zhang+5 more
doaj +1 more source
Preferential association of hepatitis C virus with CD19+ B cells is mediated by complement system [PDF]
Extrahepatic disease manifestations are common in chronic hepatitis C virus (HCV) infection. The mechanism of HCV-related lymphoproliferative disorders is not fully understood.
Alter, Harvey J.+7 more
core +1 more source