Results 41 to 50 of about 494,278 (351)

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines [PDF]

open access: yes, 2003
International audienceApoptosis was studied in the lungs of pigs during an infection with a European strain of porcine reproductive and respiratory syndrome virus (PRRSV) and it was examined if cytokines were involved in the induction of apoptosis ...
Labarque, Geoffrey   +4 more
core   +3 more sources

Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures

open access: yesVirology Journal, 2011
Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the ...
Hedlund Kjell-Olof   +8 more
doaj   +1 more source

Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery. [PDF]

open access: yes, 2010
Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery because of their safety and delivery efficiency.
Bethi, SR   +5 more
core   +2 more sources

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells [PDF]

open access: yes, 2017
Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control.
Ant, Thomas H.   +9 more
core   +3 more sources

Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]

open access: yes, 2018
Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
core   +3 more sources

Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review

open access: yes, 2020
Although HIV-1 has evolved from a deadly to a chronic disease over the past 20 years, an HIV-1 cure is still lacking due to the presence of persisting cellular viral reservoirs which are spread throughout the body in different anatomical compartments ...
De Scheerder, Marie-Angélique   +3 more
core   +1 more source

Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]

open access: yes, 2016
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart   +13 more
core   +1 more source

An Improved Workflow for the Quantification of Orthohantavirus Infection Using Automated Imaging and Flow Cytometry

open access: yesViruses
Determination of the infectious titer is a central requirement when working with pathogenic viruses. The plaque or focus assay is a commonly used but labor- and time-consuming approach for determining the infectious titer of orthohantavirus samples.
Laura Menke, Christian Sieben
doaj   +1 more source

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

open access: yesVeterinární Medicína, 2009
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic.
K. Rosenbergova   +5 more
doaj   +1 more source

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards

open access: yesAnimals, 2021
The nervous necrosis virus (NNV) is a threat to fish aquaculture worldwide, especially in Mediterranean countries. Fast and accurate diagnosis is essential to control it, and viral quantification is required to predict the level of risk of new viral ...
José G. Olveira   +3 more
doaj   +1 more source

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