Results 51 to 60 of about 494,278 (351)

Viral delivery of antioxidant genes as a therapeutic strategy in experimental models of amyotrophic lateral sclerosis. [PDF]

, 2013
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder with no effective treatment to date. Despite its multi-factorial aetiology, oxidative stress is hypothesized to be one of the key pathogenic mechanisms.
Azzouz, M, Higginbottom, A, Nanou, A, Ning, K, Shaw, P, Valori, CF, Wyles, M   +6 more
core   +1 more source

Visualization of Viral Infection Dynamics in a Unicellular Eukaryote and Quantification of Viral Production Using Virus Fluorescence in situ Hybridization

Frontiers in Microbiology, 2020
One of the major challenges in viral ecology is to assess the impact of viruses in controlling the abundance of specific hosts in the environment. To this end, techniques that enable the detection and quantification of virus-host interactions at the ...
Yaiza M. Castillo, Marta Sebastián, Marta Sebastián, Irene Forn, Nigel Grimsley, Sheree Yau, Sheree Yau, Cristina Moraru, Dolors Vaqué   +8 more
doaj   +1 more source

Simultaneous Giant Virus and Virophage Quantification Using Droplet Digital PCR

Viruses, 2022
Viruses are an abundant component of aquatic systems, but their detection and quantification remain a challenge. Virophages co-replicate with giant viruses in the shared host cell, and can inhibit the production of new giant virus particles, thereby increasing the survival of the infected host population. Here, we present a protocol for Droplet Digital
Ana del Arco, Matthias Fischer, Lutz Becks   +2 more
openaire   +5 more sources

Quantification of T4-Like and T7-Like Cyanophages Using the Polony Method Show They Are Significant Members of the Virioplankton in the North Pacific Subtropical Gyre

Frontiers in Microbiology, 2020
The North Pacific Subtropical Gyre (NPSG) is one of the largest biomes on Earth, with the cyanobacterium Prochlorococcus being the most abundant primary producer year-round.
Svetlana Goldin, Yotam Hulata, Nava Baran, Debbie Lindell   +3 more
doaj   +1 more source

Rift Valley Fever Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2019
AbstractRift Valley fever virus (RVFV) is an arthropod‐borne, zoonotic disease endemic to sub‐Saharan Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever have had up to 100% mortality rates in fetal and neonatal sheep. Upon infection of ruminant and human hosts alike, RVFV infection causes an at times severe hepatitis and pathology in many
MaRyka R, Smith, Erin E, Schirtzinger, William C, Wilson, A Sally, Davis   +3 more
openaire   +3 more sources

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

Viruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan   +3 more
doaj   +1 more source

Kinetics of acute hepatitis B virus infection in humans [PDF]

, 2001
Using patient data from a unique single source outbreak of hepatitis B virus (HBV) infection, we have characterized the kinetics of acute HBV infection by monitoring viral turnover in the serum during the late incubation and clinical phases of the ...
Brown, D., Dusheiko, G.M., Emery, V.C., Murray, J.M., Perelson, A.S., Webster, G.J.M., Whalley, S.A.   +6 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Quantitative Flow Cytometry to Measure Viral Production Using Infectious Pancreatic Necrosis Virus as a Model: A Preliminary Study

Applied Sciences, 2018
In recent decades, flow cytometry (FCM) has become an important tool in virology, due to its applications in viral replication and viral-cell interactions, as well as its capacity to quantify proteins (qFCM).
Diego Vázquez, Carmen López-Vázquez, José G. Olveira, Isabel Bandín, Carlos P. Dopazo   +4 more
doaj   +1 more source