Results 51 to 60 of about 3,645,587 (361)

Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate [PDF]

open access: yes, 2015
During the late stages of the HIV-1 replication cycle, the viral polyprotein Pr55Gag is recruited to the plasma membrane (PM), where it binds phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and directs HIV-1 assembly.
Balasubramaniam   +88 more
core   +1 more source

Quantification of T4-Like and T7-Like Cyanophages Using the Polony Method Show They Are Significant Members of the Virioplankton in the North Pacific Subtropical Gyre

open access: yesFrontiers in Microbiology, 2020
The North Pacific Subtropical Gyre (NPSG) is one of the largest biomes on Earth, with the cyanobacterium Prochlorococcus being the most abundant primary producer year-round.
Svetlana Goldin   +3 more
doaj   +1 more source

An Improved Workflow for the Quantification of Orthohantavirus Infection Using Automated Imaging and Flow Cytometry

open access: yesViruses
Determination of the infectious titer is a central requirement when working with pathogenic viruses. The plaque or focus assay is a commonly used but labor- and time-consuming approach for determining the infectious titer of orthohantavirus samples.
Laura Menke, Christian Sieben
doaj   +1 more source

Heparan sulfate proteoglycan is an important attachment factor for cell entry of Akabane and Schmallenberg viruses [PDF]

open access: yes, 2017
Akabane (AKAV) and Schmallenberg (SBV) viruses are Orthobunyavirus transmitted by arthropod vectors with a broad cellular tropism in vitro as well as in vivo Both AKAV and SBV cause arthrogryposis-hydranencephaly syndrome in ruminants.
Horimoto, Taisuke   +6 more
core   +2 more sources

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

open access: yesVeterinární Medicína, 2009
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic.
K. Rosenbergova   +5 more
doaj   +1 more source

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards

open access: yesAnimals, 2021
The nervous necrosis virus (NNV) is a threat to fish aquaculture worldwide, especially in Mediterranean countries. Fast and accurate diagnosis is essential to control it, and viral quantification is required to predict the level of risk of new viral ...
José G. Olveira   +3 more
doaj   +1 more source

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines [PDF]

open access: yes, 2003
International audienceApoptosis was studied in the lungs of pigs during an infection with a European strain of porcine reproductive and respiratory syndrome virus (PRRSV) and it was examined if cytokines were involved in the induction of apoptosis ...
Labarque, Geoffrey   +4 more
core   +3 more sources

Hepatitis C Virus: Propagation, Quantification, and Storage [PDF]

open access: yesCurrent Protocols in Microbiology, 2010
AbstractHepatitis C virus (HCV) is a leading cause of chronic liver diseases, including chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV is well known for its restricted tropism and it does not replicate well in animal species other than humans and chimpanzees. Since classical in vitro propagation of natural HCV isolates is not possible,
openaire   +3 more sources

Visualization of Viral Infection Dynamics in a Unicellular Eukaryote and Quantification of Viral Production Using Virus Fluorescence in situ Hybridization

open access: yesFrontiers in Microbiology, 2020
One of the major challenges in viral ecology is to assess the impact of viruses in controlling the abundance of specific hosts in the environment. To this end, techniques that enable the detection and quantification of virus-host interactions at the ...
Yaiza M. Castillo   +8 more
doaj   +1 more source

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

open access: yesViruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs   +3 more
doaj   +1 more source

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