Results 51 to 60 of about 3,671,396 (364)

Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]

, 2019
BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak, A Bak, Aurélie Bak, AYT Wong, BM Maghari, C Wolf, E Kamau, G Cottenet, G Cottenet, HD VanGuilder, IB Salisu, IMJ Scholtens, J Davison, JB Emerson, Joanne B. Emerson, K Arumuganathan, K Nybo, K Ramessar, L Garibyan, M Chaouachi, M Singh, M Tajadini, MA Fraiture, MJ Holden, MJ Oliver, Q Zhang, R Becker, RJ Pryor, S Broeders, S Fernandez, S Llewellyn, SA Bustin, T Demeke, W Fu, Y Hou, Y Wu   +35 more
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An Improved Workflow for the Quantification of Orthohantavirus Infection Using Automated Imaging and Flow Cytometry

Viruses
Determination of the infectious titer is a central requirement when working with pathogenic viruses. The plaque or focus assay is a commonly used but labor- and time-consuming approach for determining the infectious titer of orthohantavirus samples.
Laura Menke, Christian Sieben
doaj   +1 more source

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards

Animals, 2021
The nervous necrosis virus (NNV) is a threat to fish aquaculture worldwide, especially in Mediterranean countries. Fast and accurate diagnosis is essential to control it, and viral quantification is required to predict the level of risk of new viral ...
José G. Olveira, Sandra Souto, Isabel Bandín, Carlos P. Dopazo   +3 more
doaj   +1 more source

Effector memory differentiation increases detection of replication-competent HIV-l in resting CD4+ T cells from virally suppressed individuals. [PDF]

, 2019
Studies have demonstrated that intensive ART alone is not capable of eradicating HIV-1, as the virus rebounds within a few weeks upon treatment interruption.
Bale, Michael J, Busch, Michael P, Cox, Bryan, Deeks, Steven G, Hoh, Rebecca, Kearney, Mary F, Kulpa, Deanna A, Lackman-Smith, Carol, Maldarelli, Frank, Ptak, Roger G, Stone, Mars, Subramanian, Krupa, Wiegand, Ann, Wonderlich, Elizabeth R   +13 more
core   +1 more source

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Veterinární Medicína, 2009
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic.
K. Rosenbergova, P. Lany, Z. Pospisil, O. Kubicek, V. Celer, D. Molinkova   +5 more
doaj   +1 more source

Quantification of T4-Like and T7-Like Cyanophages Using the Polony Method Show They Are Significant Members of the Virioplankton in the North Pacific Subtropical Gyre

Frontiers in Microbiology, 2020
The North Pacific Subtropical Gyre (NPSG) is one of the largest biomes on Earth, with the cyanobacterium Prochlorococcus being the most abundant primary producer year-round.
Svetlana Goldin, Yotam Hulata, Nava Baran, Debbie Lindell   +3 more
doaj   +1 more source

Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review

, 2020
Although HIV-1 has evolved from a deadly to a chronic disease over the past 20 years, an HIV-1 cure is still lacking due to the presence of persisting cellular viral reservoirs which are spread throughout the body in different anatomical compartments ...
De Scheerder, Marie-Angélique, Depelseneer, Bram, Vandekerckhove, Linos, Wim, Trypsteen   +3 more
core   +1 more source

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells [PDF]

, 2017
Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control.
Ant, Thomas H., Charles, Philip D., Dowle, Adam A., Geoghegan, Vincent, Hester, Svenja, Larson, Tony, Rainey, Stephanie M., Sinkins, Steven P., Stainton, Kirsty, Thomas, Benjamin   +9 more
core   +3 more sources

Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery. [PDF]

, 2010
Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery because of their safety and delivery efficiency.
Bethi, SR, Gujraty, K, Jang, J-H, Kane, RS, Koerber, JT, Schaffer, DV   +5 more
core   +2 more sources

Hepatitis C Virus: Propagation, Quantification, and Storage [PDF]

Current Protocols in Microbiology, 2010
AbstractHepatitis C virus (HCV) is a leading cause of chronic liver diseases, including chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV is well known for its restricted tropism and it does not replicate well in animal species other than humans and chimpanzees. Since classical in vitro propagation of natural HCV isolates is not possible,
openaire   +3 more sources