Results 71 to 80 of about 3,645,587 (361)

T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR

Journal of Virology, 2018
cccDNA elimination is a major goal in future curative regimens for chronic HBV patients. However, PCR-based assays for cccDNA quantification show a principally constrained specificity when high levels of input virus or replicative intermediates are ...
B. Qu, Y. Ni, Florian A. Lempp, F. Vondran, S. Urban   +4 more
semanticscholar   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

Development of a Reagent Kit for the Quantitative Determination of Hepatitis B Virus (HBV) DNA in Clinical Material by PCR with Hybridization-Fluorescence Detection

Эпидемиология и вакцинопрофилактика, 2023
Relevance. Hepatitis B virus (HBV) is one of the most common viral infections affecting people worldwide and can lead to chronic hepatitis, cirrhosis and hepatocellular carcinoma. Currently 3% of the world's population are infected with hepatitis B virus
O. N. Zhigaleva, S. G. Mardanly, T. Yu. Gashenko, I. I. Ermolaev   +3 more
doaj   +1 more source

Identification of a WNT5A-Responsive Degradation Domain in the Kinesin Superfamily Protein KIF26B. [PDF]

, 2018
Noncanonical WNT pathways function independently of the β-catenin transcriptional co-activator to regulate diverse morphogenetic and pathogenic processes.
Choi, Shannon S, Cohen, Michael, Fierro, Fernando A, Ho, Hsin-Yi Henry, Hum, Jennie, Karuna, Edith P, Scales, Michael K   +6 more
core   +2 more sources

Quantification of Torque Teno Virus and Epstein-Barr Virus Is of Limited Value for Predicting the Net State of Immunosuppression After Lung Transplantation

Open Forum Infectious Diseases, 2018
Background Major hurdles for survival after lung transplantation are rejections and infectious complications. Adequate methods for monitoring immune suppression status are lacking.
R. Nordén, J. Magnusson, A. Lundin, Ka-Wei Tang, S. Nilsson, M. Lindh, L. Andersson, G. Riise, J. Westin   +8 more
semanticscholar   +1 more source

QUANTIFICATION OF WEST NILE VIRUS IN VECTOR MOSQUITO SALIVA [PDF]

Journal of the American Mosquito Control Association, 2005
Saliva was collected from 4 species of mosquitoes intrathoracically inoculated with West Nile virus (WNV). The amount of infectious virus in the saliva was quantified by plaque assay and the number of WNV genomic equivalents (GE) was measured by reverse transcriptase-polymerase chain reaction.
Alicia Johnson, Leah Colton, Roger S. Nasci, Brad J. Biggerstaff   +3 more
openaire   +2 more sources

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

Neuronal activity regulates neurotransmitter switching in the adult brain following light-induced stress. [PDF]

, 2018
Neurotransmitter switching in the adult mammalian brain occurs following photoperiod-induced stress, but the mechanism of regulation is unknown. Here, we demonstrate that elevated activity of dopaminergic neurons in the paraventricular nucleus of the ...
Deisseroth, Karl, Leutgeb, Stefan, Li, Hui-Quan, Meng, Da, Spitzer, Nicholas C   +4 more
core   +1 more source

Quantification of Adeno-Associated Virus with Safe Nucleic Acid Dyes [PDF]

Human Gene Therapy, 2020
AbstractAdeno-associated virus (AAV) is the most commonly used viral vector for both biological and gene therapeutic applications1. Although many methods have been developed to measure quantity attributes of AAV, they are often technically challenging and time consuming. Here we report a method to titer AAV with GelGreen® dye, a safe green fluorescence
Xu, Jian, DeVries, Steven H, Zhu, Yongling   +2 more
openaire   +4 more sources

TOMM20 as a driver of cancer aggressiveness via oxidative phosphorylation, maintenance of a reduced state, and resistance to apoptosis

Molecular Oncology, EarlyView.
TOMM20 increases cancer aggressiveness by maintaining a reduced state with increased NADH and NADPH levels, oxidative phosphorylation (OXPHOS), and apoptosis resistance while reducing reactive oxygen species (ROS) levels. Conversely, CRISPR‐Cas9 knockdown of TOMM20 alters these cancer‐aggressive traits.
Ranakul Islam, Megan E. Roche, Zhao Lin, Diana Whitaker‐Menezes, Victor Diaz‐Barros, Eurico Serrano, Maria Paula Martinez Cantarin, Nancy J. Philp, Atrayee Basu Mallick, Ubaldo Martinez‐Outschoorn   +9 more
wiley   +1 more source