Quantification of Adeno-Associated Virus with Safe Nucleic Acid Dyes [PDF]
Human Gene Therapy, 2020 AbstractAdeno-associated virus (AAV) is the most commonly used viral vector for both biological and gene therapeutic applications1. Although many methods have been developed to measure quantity attributes of AAV, they are often technically challenging and time consuming. Here we report a method to titer AAV with GelGreen® dye, a safe green fluorescence
Xu, Jian +2 more
openaire +4 more sources
FEBS Letters, EarlyView.Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu +17 more
wiley +1 more source
Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
, 2018 Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
core +3 more sources
From omics to AI—mapping the pathogenic pathways in type 2 diabetes
FEBS Letters, EarlyView.Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan +2 more
wiley +1 more source
Quantification and a Molecular Dynamics Study of Viral Membrane Lipids through Plasmon Coupling Microscopy [PDF]
, 2015 Phosphatidylserine (PS) and monosialotetrahexosylganglioside (G_M1) are examples of two host-derived lipids in the membrane of enveloped virus particles that are known to contribute to virus attachment, uptake, and ultimately dissemination.
Feizpour, Amin, Reinhard, Björn
core +1 more source
Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]
, 2016 Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart +13 more
core +1 more source
Hepatitis C virus quantification in serum and saliva of HCV-infected patients.
Memórias do Instituto Oswaldo Cruz, 2012 The hepatitis C virus (HCV) can be detected in blood and other bodily fluids, such as saliva, semen and gastric juices. The aim of this study was to compare the HCV viral loads in the serum and saliva of infected patients.
G. Menezes +8 more
semanticscholar +1 more source
ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling
FEBS Letters, EarlyView.In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li +3 more
wiley +1 more source
Clinical Utility of Quantitative Hbsag in Chronic Hepatitis B Infection
The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy, 2020 The prevalence of hepatitis B infection in Indonesia is still alarming, while its therapies cannot eradicate the virus and only aim for long term viral suppression.
Poernomo Boedi Setiawan +2 more
doaj +1 more source
Human papillomavirus E2 regulates SRSF3 (SRp20) to promote capsid protein expression in infected differentiated keratinocytes [PDF]
, 2016 The human papillomavirus (HPV) life cycle is tightly linked to differentiation of the infected epithelial cell suggesting a sophisticated interplay between host cell metabolism and virus replication.
Beemon, K. L. +5 more
core +2 more sources