Results 31 to 40 of about 1,229 (262)

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Study on the Injection Modes and Displacement Characteristics of Chemical Compound Flooding in Heavy Oil Reservoirs After Multiple Cycles of Huff-and-Puff

Energies
The chemical agent injection modes and displacement characteristics of chemical compound flooding, consisting of a plugging agent, an oil displacement agent, and a viscosity reducer, were investigated by laboratory experiments for target heavy oil ...
Li Zhang, Lei Tao, Guanli Xu, Jiajia Bai
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Pore-Scale Mechanism Analysis of Enhanced Oil Recovery by Horizontal Well, Dissolver, Nitrogen, and Steam Combined Flooding in Reducer Systems with Different Viscosities for Heavy Oil Thermal Recovery

Energies
Horizontal well, dissolver, nitrogen, and steam (HDNS) combined flooding is mainly applied to shallow and thin heavy oil reservoirs to enhance oil recovery. Due to the lack of pore-scale mechanism studies, it is impossible to clarify the oil displacement
Bowen Zhang, Zhiyong Song, Yang Zhang
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Horizontal well, nitrogen and viscosity reducer assisted steam huff and puff technology: Taking super heavy oil in shallow and thin beds, Chunfeng Oilfield, Junggar Basin, NW China, as an example

Petroleum Exploration and Development, 2013
Chunfeng Oilfield in the Junggar Basin has shallow burial depth (400–570 m), low formation temperature (22–28 °C), thin reservoirs (2–6 m, averaging 3.5 m), high underground crude oil viscosity (50 000–90 000 mPa·s), but high sensitivity to heat, it is ...
Xuezhong WANG, Jinzhu WANG, Mingquan QIAO   +2 more
doaj   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Settling of Spherical Particles in High Viscosity Friction Reducer Fracture Fluids

Energies, 2021
Investigating the key factors that impact fluid rheology and proppant static settling velocity in high viscosity friction reducers (HVFRs) is a critical aspect for successful proppant transport in hydraulic fracture treatment.
Ghith Biheri, Abdulmohsin Imqam
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Light‐Assisted 3D Printing Techniques and Photocrosslinking Strategies: Recent Advances in Musculoskeletal Tissue Engineering

Advanced Engineering Materials, EarlyView.
In this review, the current state of light‐assisted 3D printing as it pertains to engineering musculoskeletal tissues including bone, cartilage, skeletal muscle, tendon, and ligaments is summarized. Common printing techniques, photoreactive materials, and study design choices are compiled and reviewed.
Meagan Morgan, Bin Zhang, Roger Narayan
wiley   +1 more source