Results 51 to 60 of about 313,022 (252)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Valdur Mikita and the return of the real: In search of any-spaces-whatever in Estonian forests

open access: yesSign Systems Studies
Around the turn of the millennium, a paradigm shift took place in Western arts and humanities. (Post)structuralism, which had focused on language, the signifier, the epistemological, receded, and both academic and artistic attention turned to the real ...
Sven Vabar
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

open access: yesFEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart   +7 more
wiley   +1 more source

La obra de arte en la época del colapso medioambiental: fricciones, paradojas y bifurcaciones

open access: yesMayéutica: Revista Científica de Humanidades y Artes
Este artículo examina algunas paradojas presentes en las prácticas artísticas, el lenguaje y la narración comprometidos con el ambientalismo, a la vez que explora posibles bifurcaciones capaces de desafiar las actuales visiones hegemónicas del mundo ...
Jye O’ Sullivan, Ester Toribio-­Roura
doaj   +1 more source

A few remarks on the mechanisms of censorship in the PRL and the Third Republic of Poland

open access: yesActa Universitatis Lodziensis. Folia Litteraria Polonica, 2017
The purpose of the paper is to discuss the mechanism of censorship before and after 1990 when the mission of the communist Chief Control Bureau for Press, Publications and Performances (GUKPPiW) was terminated.
Jakub Dąbrowski
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Developing the ArchAIDE Application: A digital workflow for identifying, organising and sharing archaeological pottery using automated image recognition

open access: yesInternet Archaeology, 2020
Pottery is of fundamental importance for understanding archaeological contexts, facilitating the understanding of production, trade flows, and social interactions.
Francesca Anichini   +21 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

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