Results 41 to 50 of about 668,131 (266)

Nanozyme-assisted amplification-free CRISPR/Cas system realizes visual detection

Frontiers in Bioengineering and Biotechnology
The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated) system has proven to be a powerful tool for nucleic acid detection due to its inherent advantages of effective nucleic acid identification and editing ...
Yuan Zhang, Wanpeng Yu, Man Wang, Lei Zhang, Peifeng Li   +4 more
doaj   +1 more source

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Viruses, 2022
A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu, Lulu Zuo, Jing Miao, Lingjing Mao, Benjamin Selekon, Ella Gonofio, Emmanuel Nakoune, Nicolas Berthet, Gary Wong   +8 more
doaj   +1 more source

Visual Detection of Cysteine and Homocysteine

Journal of the American Chemical Society, 2003
The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color ...
Oleksandr, Rusin, Nadia N, St Luce, Rezik A, Agbaria, Jorge O, Escobedo, Shan, Jiang, Isiah M, Warner, Fareed B, Dawan, Kun, Lian, Robert M, Strongin   +8 more
openaire   +3 more sources

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Rapid and reliable diagnosis of Moraxella catarrhalis infection using loop-mediated isothermal amplification-based testing

Frontiers in Bioengineering and Biotechnology
Moraxella catarrhalis (M. catarrhalis) was an important pathogen closely associated with respiratory tract infections. We employed the loop-mediated isothermal amplification (LAMP) coupled with nanoparticle-based lateral flow biosensor (LFB) and ...
Fei Xiao, Juan Zhou, Xiaolan Huang, Jin Fu, Nan Jia, Chunrong Sun, Zheng Xu, Yi Wang, Lei Yu, Lihui Meng   +9 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

A Comparative Study on Visual Detection of Mycobacterium tuberculosis by Closed Tube Loop-Mediated Isothermal Amplification: Shedding Light on the Use of Eriochrome Black T

Diagnostics, 2023
Loop-mediated isothermal amplification is a promising candidate for the rapid detection of Mycobacterium tuberculosis. However, the high potential for carry-over contamination is the main obstacle to its routine use. Here, a closed tube LAMP was intended
Alireza Neshani, Hosna Zare, Hamid Sadeghian, Hadi Safdari, Bamdad Riahi-Zanjani, Ehsan Aryan   +5 more
doaj   +1 more source

Keyframe detection in visual lifelogs

Proceedings of the 1st international conference on PErvasive Technologies Related to Assistive Environments, 2008
The SenseCam is a wearable camera that passively captures images. Therefore, it requires no conscious effort by a user in taking a photo. A Visual Diary from such a source could prove to be a valuable tool in assisting the elderly, individuals with neurodegenerative diseases, or other traumas. One issue with Visual Lifelogs is the large volume of image
Blighe, Michael, Doherty, Aiden R., Smeaton, Alan F., O'Connor, Noel E.   +3 more
openaire   +2 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Visual Detection and Image Processing of Parking Space Based on Deep Learning

Sensors, 2022
The automatic parking system based on vision is greatly affected by uneven lighting, which is difficult to make an accurate judgment on parking spaces in the case of complex image information.
Chen Huang, Shiyue Yang, Yugong Luo, Yongsheng Wang, Ze Liu   +4 more
doaj   +1 more source