Results 101 to 110 of about 23,516 (259)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Geometric entropy of plant leaves: A measure of morphological complexity.

PLoS ONE
Shape is an objective characteristic of an object. A boundary separates a physical object from its surroundings. It defines the shape and regulates energy flux into and from an object.
Vishnu Muraleedharan, Sajeev C Rajan, Jaishanker R   +2 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Pictures and interpretations : towards an applied semiotics

, 2012
This is a study about the ways in which pictures can be interpreted and the ways in which they are interpreted; the latter, specifically, in a relatively remote part of Peru.
Boot, Katie
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A comparison of visual- and stereo-video based fish community assessment methods in tropical and temperate marine waters of Western Australia

, 2013
Robust assessments of abundance and diversity are essential components of research programs aimed at detecting changes in marine fish assemblages through space and time.
Evans, R., Shedrawi, G., Travers, M., Douglas, R., wilson, S., Hickey, K., Moore, G., Langlois, T., Harvey, Euan, holmes, T.   +9 more
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Analysis Of The Mangrove Structure In The Dong Rui Commune Based On Multispectral Unmanned Aerial Vehicle Image Data

Geography, Environment, Sustainability
Mangroves are one of the most important types of wetlands in coastal areas and perform many different functions. Assessing the structure and function of mangroves is a premise for the management, monitoring and development of this most diverse and ...
D. T. Ngo, K. N. Quoc, N. T. Dang, C. H. Dang, L. L. Tran, H. D. Nguyen   +5 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

eDNA metabarcoding warms up a hotspot of marine biodiversity: revealing underrepresented taxa in visual surveys and historical records from the Gulf of California

Environmental DNA (eDNA) metabarcoding is revolutionizing biodiversity monitoring, but comparisons against traditional data rarely include long-term historical inventories.
Valdivia-Carrillo, Tania, Valenzuela-Quiñonez, Fausto, Brusca, Richard C., Reyes-Bonilla, Hector, Munguia-Vega, Adrian, Mac Loughlin, Camila   +5 more
core   +1 more source