Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Electrothermally Addressable Liquid Crystal Elastomer Microactuator Arrays with Integrated Microheaters. [PDF]
Li K, Fernandes J, Kim JJ, Jiang H.
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Thermally Stable HfO<sub>2</sub>-Based Ferroelectric Transistors for CMOS-Compatible Energy-Efficient Neuromorphic Integrated Circuits. [PDF]
Tikhonenko FV +4 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Multiphysics Analysis and Optimization of a Thin-Film Lithium Niobate Phase Modulator for Fiber-Optic Gyroscopes. [PDF]
Zhang H +6 more
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Dynamically Tunable Exciton-Photon Coupling via Multi-Physics Field in Transition Metal Dichalcogenide Microcavities. [PDF]
Zhang J +7 more
europepmc +1 more source
Successful Extensive Pulsed Field Ablation for Atrial Fibrillation Without Leadless Pacemaker Interference. [PDF]
Matsuura G +4 more
europepmc +1 more source
A novel hybrid SEPIC-Landsman bidirectional converter for renewable-energy-based EV charging. [PDF]
S H +3 more
europepmc +1 more source

