Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Volume of fluid consumption during preparation for colonoscopy is possibly the single most important determinant of bowel preparation adequacy. [PDF]
Ann Gastroenterol, 2021 Gorelik Y +5 more
europepmc +1 more source
Memory performance mediates subjective sleep quality associations with cerebrospinal fluid Alzheimer's disease biomarker levels and hippocampal volume among individuals with mild cognitive symptoms [PDF]
, 2023 Laura Stankeviciute +10 more
openalex +1 more source
Intercompartmental communication in senescence
FEBS Open Bio, EarlyView.Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz +3 more
wiley +1 more source
Amniotic Fluid and Maternal Serum Laeverin Levels and Their Correlations with Fetal Size and Placental Volume in Second Trimester of Pregnancy—A Prospective Cross-Sectional Study [PDF]
János Sikovanyecz +7 more
openalex +1 more source
Transfusion of Blood Products in Trauma Victims: Effects of Fresh Frozen Plasma, Platelet and Intravenous Fluid to Packed Red Blood Cell Volume Ratios on Survival [PDF]
, 2013 Michael C. Plewa +5 more
openalex +1 more source
On Solid-Fluid Mixture Models for High Particulate Volume Fractions [PDF]
, 2021 Andrea Acri +4 more
openalex +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source