Results 41 to 50 of about 50,745 (217)

Quantifying the impacts of groundwater abstraction on Ganges river water infiltration into shallow aquifers under the rapidly developing city of Patna, India

open access: yesJournal of Hydrology: Regional Studies, 2022
Study region: Patna is located on the southern bank of the River Ganges in Bihar, India. Rapid population growth over the past few decades has driven an increase in groundwater abstraction from aquifers under the city.
Chuanhe Lu   +8 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Performances Analysis and Comparison of Active and Hybrid Harmonic Filter in Photovoltaic Water Pumping System

open access: yesJournal of Engineering Technology and Applied Physics, 2023
As an agriculturally based country, many rivers pumping stations are implemented in Myanmar. Currently, these pumping stations are operated with utility supply or diesel engines.
Si Si Maw
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

open access: yesFEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey   +4 more
wiley   +1 more source

An assessment of capacity of Brestovica – Klariči karst water supply (Slovenia)

open access: yesActa Carsologica, 2012
In August 2008 an intensive pumping experiment was carried out in order to assess the capacity of Brestovica – Klarici water supply in summer conditions, and its chemical quality.
Janko Urbanc, Kim Mezga, Luca Zini
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Using the Resistivity Imaging Method to Monitor the Dynamic Effects on the Vadose Zone During Pumping Tests at the Pengtsuo Site in Pingtung, Taiwan

open access: yesTerrestrial, Atmospheric and Oceanic Sciences, 2016
We conducted a time-lapse monitoring study during a well-pumping test at the Pengtsuo site in Pingtung, Taiwan. Water-level gauges were installed in four wells (P1, W1, O1, and O2) at the Pengtsuo site with different screen depths for the observation. We
Ping-Yu Chang   +5 more
doaj   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

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