Results 51 to 60 of about 3,721,948 (299)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

An assessment of capacity of Brestovica – Klariči karst water supply (Slovenia)

Acta Carsologica, 2012
In August 2008 an intensive pumping experiment was carried out in order to assess the capacity of Brestovica – Klarici water supply in summer conditions, and its chemical quality.
Janko Urbanc, Kim Mezga, Luca Zini
doaj   +1 more source

Optimization Scheduling Method for Irrigation Systems with Two Reservoirs and Three Pumping Stations in Hilly Areas

Guan'gai paishui xuebao, 2023
【Objective】 This paper develops an optimal scheduling method for joint operation of two reservoirs and three pumping stations irrigation systems in hilly areas.
LUO Qihua, JIANG Xiaohong, CHEN Yu, CHENG Jilin   +3 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Using the Resistivity Imaging Method to Monitor the Dynamic Effects on the Vadose Zone During Pumping Tests at the Pengtsuo Site in Pingtung, Taiwan

Terrestrial, Atmospheric and Oceanic Sciences, 2016
We conducted a time-lapse monitoring study during a well-pumping test at the Pengtsuo site in Pingtung, Taiwan. Water-level gauges were installed in four wells (P1, W1, O1, and O2) at the Pengtsuo site with different screen depths for the observation. We
Ping-Yu Chang, Shao-Yiu Hsu, Liang-Cheng Chang, Wen-Fu Chen, Yu-Wen Chen, Hsueh-Yu Lu   +5 more
doaj   +1 more source

An Intelligent Mobile Gas Drainage Pumping Station

Meikuang Anquan, 2020
In this paper, an intelligent mobile gas pumping station is designed. By designing and installing the intelligent monitoring substation and water-softening device on the mobile gas drainage pumping station, the monitoring of various parameters in the ...
MENG Fanrui, ZHAO Yong, JING Yanbo, ZHAO Guoyong, SHAO Jirong, REN Zhichao
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

A Depolarizing Leak in Sodium Bicarbonate Cotransporter NBCe1 Causes Brain Edema

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives SLC4A4 encodes electrogenic sodium bicarbonate cotransporter NBCe1, prominently expressed in kidney and brain. Recessive loss‐of‐function variants in SLC4A4 cause proximal renal tubular acidosis, no brain edema. In the brain, NBCe1 is expressed by astrocytes, where it regulates pH and mediates astrocyte volume changes.
Quinty Bisseling, Mark D. Parker, Sven Kerst, Richard A. Pasternack, Jacob Tondreau, Marjolein Breur, Gemma M. van Rooijen‐van Leeuwen, Davide Tonduti, Ettore Salsano, Alejandra Darling, Joanna A. E. van Wijk, Susanna Törnroth‐Horsefield, Marianna Bugiani, Petra J. W. Pouwels, Quinten Waisfisz, Marjo S. van der Knaap, Rogier Min   +16 more
wiley   +1 more source

Optimization of Photovoltaic Water Pumping Systems: Progress, Limits, and Prospects for a Healthy Energy Future

Journal of Techniques
Today, water pumping plays a crucial role throughout the world, due to its many uses, such as irrigation, watering livestock and supplying water for domestic needs in isolated areas not served by traditional distribution networks (of water and/or ...
Alain Foutche Tchouli, Stephane Ngasop Ndiya, Hilaire Tchami, Claude Bertin Nzoundja Fapi, Hyacinthe Tchakounté   +4 more
doaj   +1 more source

Energy and Cost Savings through Pumping Stations Rehabilitation. Case Study in Bucharest

Proceedings, 2018
This paper presents the results recorded by upgrading and rehabilitating the pumping stations for an urban water network with a primary goal of diminishing the operation and maintenance costs and a secondary goal of reducing the water losses in the water
Sorin Perju, Alexandru Aldea
doaj   +1 more source