Results 161 to 170 of about 14,982,501 (348)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Impact of conditioners added to soil of different bulk density on saturated water content and water holding capacity over a six-day manipulative experiment

Central European Forestry Journal
Artificial superabsorbents specifically influence the water absorption capacity of soils. The article compares four commercially available synthetic superabsorbents mixed with soil of different bulk density. Soils at each of nine study sites were sampled
Špulák Ondřej, Kacálek Dušan, Bartoš Jan, Leugner Jan, Ráček Zdeněk   +4 more
doaj   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

WASP: Wearable Analytical Skin Probe for Dynamic Monitoring of Transepidermal Water Loss. [PDF]

ACS Sens, 2023
Sivakumar AD, Sharma R, Thota C, Ding D, Fan X.   +4 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Small Papillae Regulated by SPD25 are Critical for Balancing Photosynthetic CO₂ Assimilation and Water Loss in Rice. [PDF]

Rice (N Y), 2023
Zhu L, Zeng F, Liang Y, Wang Q, Chen H, Feng P, Fan M, Cheng Y, Wang J.   +8 more
europepmc   +1 more source

Sustained Water Loss in California's Mountain Ranges During Severe Drought From 2012 to 2015 Inferred From GPS

, 2017
D. Argus, F. Landerer, D. Wiese, H. Martens, Yuning Fu, J. Famiglietti, B. Thomas, T. Farr, A. Moore, M. Watkins   +9 more
semanticscholar   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Light cues induce protective anticipation of environmental water loss in terrestrial bacteria. [PDF]

Proc Natl Acad Sci U S A, 2023
Hatfield BM, LaSarre B, Liu M, Dong H, Nettleton D, Beattie GA.   +5 more
europepmc   +1 more source