Results 31 to 40 of about 925,626 (245)

Discerning protein pools by selective staining with self‐labeling tags

open access: yesFEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Wavelength Calibration for OSIRIS/GTC¹ Tunable Filters [PDF]

open access: yes, 2011
OSIRIS (Optical System for Imaging and low Resolution Integrated Spectroscopy) is the first light instrument of the Gran Telescopio Canarias (GTC). It provides a flexible and competitive tunable filter (TF).
Yalia Divakara Mayya   +4 more
core  

Calibration methods for a dual-wavelength interferometer system

open access: yes, 2018
Multiple wavelength interferometry has long been considered an option for the measurement of large aspheric slope departures. In particular, a synthetic wavelength offers a variety of approaches by which large phase excursions can be unwrapped.
Tangari Larrategui, Martin   +7 more
core   +1 more source

Airborne Visible/Infrared Imaging spectrometer AVIS: Design, characterization and calibration [PDF]

open access: yes, 2007
The Airborne Visible/Infrared imaging Spectrometer AVIS is a hyperspectral imager designed for environmental monitoring purposes. The sensor, which was constructed entirely from commercially available components, has been successfully deployed during ...
Mauser, Wolfram   +3 more
core   +1 more source

FIMS Wavelength Calibration via Airglow Line Observations

open access: yesJournal of Astronomy and Space Sciences, 2004
Far-ultraviolet Imaging Spectrograph (FIMS) is the main payload of the Korea's first scientific micro satellite STSAT-1, which was launched at Sep. 27 2003 successfully.
Dae-Hee Lee   +10 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Preflight Spectral Calibration of Airborne Shortwave Infrared Hyperspectral Imager with Water Vapor Absorption Characteristics

open access: yesSensors, 2019
Due to the strong absorption of water vapor at wavelengths of 1350–1420 nm and 1820–1940 nm, under normal atmospheric conditions, the actual digital number (DN) response curve of a hyperspectral imager deviates from the Gaussian shape, which ...
Honglin Liu, Dong Zhang, Yueming Wang
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Calibration-free wavelength modulation spectroscopy based on even-order harmonics

open access: yes, 2021
This paper proposes a novel and rapid calibration-free wavelength modulation spectroscopy algorithm based on even-order harmonics. The proposed algorithm, analytically deduced from Voigt line-shape function, only involves simple algebraic operations to ...
Yihong Wang (205989)   +2 more
core   +1 more source

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