Results 111 to 120 of about 607,594 (307)

The Chills and Thrills of Whole Genome Sequencing

CoRR, 2013
A slightly different version of this article appears in IEEE Computer Magazine, Vol. 48, No. 2, February 2015, under the title "Whole Genome Sequencing: Revolutionary Medicine or Privacy Nightmare"
Erman Ayday, Emiliano De Cristofaro, Jean-Pierre Hubaux, Gene Tsudik   +3 more
openaire   +2 more sources

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

FinaleMe: Predicting DNA methylation by the fragmentation patterns of plasma cell-free DNA

Nature Communications
Analysis of DNA methylation in cell-free DNA reveals clinically relevant biomarkers but requires specialized protocols such as whole-genome bisulfite sequencing.
Yaping Liu, Sarah C. Reed, Christopher Lo, Atish D. Choudhury, Heather A. Parsons, Daniel G. Stover, Gavin Ha, Gregory Gydush, Justin Rhoades, Denisse Rotem, Samuel Freeman, David W. Katz, Ravi Bandaru, Haizi Zheng, Hailu Fu, Viktor A. Adalsteinsson, Manolis Kellis   +16 more
doaj   +1 more source

Identifying insertion mutations by whole-genome sequencing

BioTechniques, 2011
Insertion mutagenesis via mobile genetic element is a common technique for the analysis of gene function in model organisms. Next-generation sequencing offers an attractive approach for localizing the site of insertion, but alignment-based mapping of ...
Harold E. Smith
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Novel approach for deriving genome wide SNP analysis data from archived blood spots [PDF]

, 2012
BACKGROUND: The ability to transport and store DNA at room temperature in low volumes has the advantage of optimising cost, time and storage space.
Walling, Grant A, Fowler Katie E, Fowler, K., Fowler, Katie E., Griffin, Darren K, Griffin, D., Reitter, Chris P, Reitter Chris P, Griffin Darren K, Griffin, Darren K., Reitter, C., Walling, G., Walling Grant A   +12 more
core   +1 more source

Mapping Challenging Mutations by Whole-Genome Sequencing

G3: Genes, Genomes, Genetics, 2016
Whole-genome sequencing provides a rapid and powerful method for identifying mutations on a global scale, and has spurred a renewed enthusiasm for classical genetic screens in model organisms. The most commonly characterized category of mutation consists
Harold E. Smith, Amy S. Fabritius, Aimee Jaramillo-Lambert, Andy Golden   +3 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Whole-genome sequencing to establish relapse or re-infection with Mycobacterium tuberculosis : a retrospective observational study

, 2013
This study was funded by Wellcome Trust (grant number 098051), European Union (FP7 grant number 241 476), Medical Research Council, Global Alliance for TB Drug Development, and European and Developing Country Clinical Trials Partnership (grants IP.2007 ...
van Helden, Paul, Bryant, J.M., Bentley, S.D., Louw, Cheryl, Bateson, Anna L C, Boeree, M.J., Chuchottaworn, Charoen, Mahayiddin, Aziah A, Hoelscher, Michael, Gillespie, Stephen Henry, Mwaigwisya, Solomon, Parkhill, J., Bentley, Stephen D, Pym, Alex, Ahmad Mahayiddin, Aziah, Boeree, Martin J, Diacon, Andreas, Harris, Simon R, Harris, S.R., McHugh, Timothy D, Bryant, Josephine M, Chuchottaworn, C, Wright, Laura, Parkhill, Julian, Hunt, Robert D, Dawson, Rodney, Sanne, Ian M, Gillespie, Stephen H, Diacon, Andreas H   +28 more
core   +1 more source

Whole genome sequencing in paediatric channelopathy and cardiomyopathy

Frontiers in Cardiovascular Medicine
BackgroundPrecision medicine in paediatric cardiac channelopathy and cardiomyopathy has a rapid advancement over the past years. Compared to conventional gene panel and exome-based testing, whole genome sequencing (WGS) offers additional coverage at the ...
Sit Yee Kwok, Anna Ka Yee Kwong, Julia Zhuo Shi, Connie Fong Ying Shih, Mianne Lee, Christopher C. Y. Mak, Martin Chui, Sabrina Tsao, Brian Hon Yin Chung   +8 more
doaj   +1 more source