Results 121 to 130 of about 652,594 (298)

nek-workflow: GlobusWorld 2015

, 2015
<p>This is the state of nek-workflow as of GlobusWorld 2015.</p ...
Max Hutchinson
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

An Evaluation of Inter-Organizational Workflow Modelling Formalisms [PDF]

This paper evaluates the dynamic aspects of the UML in the context of inter-organizational workflows. Two evaluation methodologies are used. The first one is ontological and is based on the BWW (Bunge-Wand-Weber) models. The second validation is based on
Michel Patry, Benoit A. Aubert, Aymeric Dussart   +2 more
core  

Galaxy-Workflow-De_Novo_Assembly_workflow_for_dual_hepatitis_E_virus_infection.ga

, 2018
This workflow takes paired fastq files, trims and normalises them and assemblies the output to produce contigs, which will be identified by BLAST afterwards.
Juan Ledesma (5923691)
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

common-workflow-language/cwltool 1.0.20170928192020

, 2017
Common Workflow Language reference ...
Thomas Hickman, John Kern, Kapil kumar, Brad Chapman, Abram Connelly, psaffrey-illumina, Tomoya Tanjo, Denis Yuen, Janneke van der Zwaan, Andrey Kartashov, Gijs Molenaar, Manvendra Singh, Guillermo Carrasco, Alejandro Barrera, Michael Kotliar, Ward Vandewege, Dan Leehr, Stian Soiland-Reyes, Hervé Ménager, John Bradley, Sinisa Ivkovic, John Chilton, Michael R. Crusoe, boysha, Peter Amstutz, Joshua C. Randall, Nebojsa Tijanic, Pau Ruiz Safont, Ryan Spangler, Jiayong Li, James J Porter, Radhika Chippada   +31 more
core   +1 more source

Evo‐Scope: Fully automated assessment of correlated evolution on phylogenetic trees

Methods in Ecology and Evolution
Correlated evolution describes how multiple biological traits evolve together. Recently developed methods provide increasingly detailed results of correlated evolution, sometimes at elevated computational costs.
Maxime Godfroid, Charles Coluzzi, Amaury Lambert, Philippe Glaser, Eduardo P. C. Rocha, Guillaume Achaz   +5 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source