Results 51 to 60 of about 523,530 (315)

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Alpha shapes: determining 3D shape complexity across morphologically diverse structures

BMC Evolutionary Biology, 2018
Background Following recent advances in bioimaging, high-resolution 3D models of biological structures are now generated rapidly and at low-cost. To use this data to address evolutionary and ecological questions, an array of tools has been developed to ...
James D. Gardiner, Julia Behnsen, Charlotte A. Brassey   +2 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Nano Organic Co‐Crystal Scintillator for X‐ray Imaging

Small Structures, 2023
Traditional‐metal‐containing scintillators are widely used in X‐ray imaging due to their efficient X‐ray absorption and output of visible light. However, they suffer from heavy‐metal toxicity, environmental stability, harsh preparation, and afterglow ...
Qisheng Sun, Hongyun Wang, Jing Li, Fei Li, Weigang Zhu, Xiaotao Zhang, Qiushui Chen, Huanghao Yang, Wenping Hu   +8 more
doaj   +1 more source

Crystal-Based X-ray Interferometry and Its Application to Phase-Contrast X-ray Imaging, Z_eff Imaging, and X-ray Thermography

Applied Sciences, 2023
Crystal-based X-ray interferometry (CXI) detects X-ray phase shifts by using the superposition of waves, and its sensitivity is the highest among the other X-ray phase-detecting methods.
Akio Yoneyama, Daiko Takamatsu, Thet-Thet Lwin, Shigehito Yamada, Tetsuya Takakuwa, Kazuyuki Hyodo, Keiichi Hirano, Satoshi Takeya   +7 more
doaj   +1 more source

First application experiments with the Stockholm compact soft x-ray microscope

, 2022
Most soft x-ray microscopes operating in the water window (λ = 2.3 − 4.4 nm) rely on synchrotron radiation sources. In the future we believe scientists will use soft x-ray microscopes as one imaging tool among others in their own laboratory.
Vogt, U., von Hofsten, O., J Thieme, ; 2Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, D-37077 Göttingen, Germany, Holmberg, A., Hertz, H., O von Hofsten, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, H Hertz, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, Lindblom, M., A Holmberg, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, M Bertilson, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, Bertilson, M., U Vogt, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, Takman, P., P Takman, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden, Thieme, J., M Lindblom, ; 1Biomedical and X-Ray Physics, Royal Institute of Technology/Albanova, SE-10691, Stockholm, Sweden   +15 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Towards time-resolved multiscale and multimodal imaging

npj Imaging
Current biomedical imaging focuses on spatial detail but overlooks time, limiting our understanding of disease progression. There is an unmet need for temporal atlases that align multiscale and multimodal data across defined timepoints, enabling dynamic ...
Jishizhan Chen
doaj   +1 more source

Polymer composite microtube array produced by meniscus-guided approach

AIP Advances, 2013
Single freestanding microtubes of poly(methyl methacrylate)/polypyrrole (PMMA/PPy) are produced based on a meniscus-guided approach. A ring-deposit of nanoparticles is first formed in a meniscus solution of PMMA/PPy nanoparticles by outward liquid flow ...
Kyu Hwang Won, Byung Mook Weon, Jung Ho Je   +2 more
doaj   +1 more source

Novel System for Real-Time Integration of 3-D Echocardiography and Fluoroscopy for Image-Guided Cardiac Interventions: Preclinical Validation and Clinical Feasibility Evaluation

IEEE Journal of Translational Engineering in Health and Medicine, 2014
Real-time imaging is required to guide minimally invasive catheter-based cardiac interventions. While transesophageal echocardiography allows for high-quality visualization of cardiac anatomy, X-ray fluoroscopy provides excellent visualization of devices.
Aruna V. Arujuna, R. James Housden, Yingliang Ma, Ronak Rajani, Gang Gao, Niels Nijhof, Pascal Cathier, Roland Bullens, Geert Gijsbers, Victoria Parish, Stamatis Kapetanakis, Jane Hancock, C. Aldo Rinaldi, Michael Cooklin, Jaswinder Gill, Martyn Thomas, Mark D. O'neill, Reza Razavi, Kawal S. Rhode   +18 more
doaj   +1 more source