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The Physiology of the Xenopus laevis Ovary
2006Xenopus laevis has been used for many decades to study oocyte development and maturation. The Xenopus oocytes' large size, relative abundance, and clearly defined progression of physical characteristics from oogonia to eggs make them ideal for studying oogenesis.
Melissa A, Rasar, Stephen R, Hammes
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Immunoglobulin classes in Xenopus laevis
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 19741. 1. Electrophoretically pure 19 S and 7 S immunoglobulins, designated IgMand IgG, were prepared from Xenopus laevis serum. Both immunoglobulins possessed antibody activity. 2. 2. The sedimentation coefficient for IgM is 19·5 S, for IgG 6·1 S. Reduction and alkylation of IgM yielded subunits with a sedimentation coefficient of 4·1 S. 3. 3.
R D, Jurd, G T, Stevenson
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Antigens of the lens of Xenopus laevis
Experimental Eye Research, 1968The antigens of the lens of Xenopus laevis were investigated by elect rophoresis, immunological double-diffusion methods and immuno-electrophoresis. Twenty-two lens antigens were detected. A new nomenclature for lens proteins is suggested.
J C, Campbell, R M, Clayton, D E, Truman
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Alternative housing for Xenopus laevis
Lab Animal, 2008At the authors' facility, housing arrangements for Xenopus laevis were cumbersome and labor-intensive, requiring technicians to wash frog tanks by hand several times a week. The authors describe an alternative housing solution they implemented by modifying a rack system that was originally used to maintain zebrafish.
Jaime, White-James +3 more
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FGFR3 expression in Xenopus laevis
Gene Expression Patterns, 2010We studied the expression of FGF receptor 3 (FGFR3) mRNA throughout early development of Xenopus laevis by RT-PCR and in situ hybridization. RT-PCR shows that FGFR3 mRNA is localized within the gastrula; regionalized staining is detected by the neural plate stage and continues throughout embryonic development.
Pope, Amanda Popielski +3 more
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The spindle checkpoint in Xenopus Laevis
Frontiers in Bioscience, 2008The spindle checkpoint ensures accurate chromosome segregation by delaying anaphase onset until all kinetochores have properly established bipolar attachment to spindle microtubules. This mechanism is important for all eukaryotic cells and is evolutionarily conserved.
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Raman spectroscopy of Xenopus laevis oocytes
Methods, 2010This work reports on the application of Raman spectroscopy for the analysis of Xenopus laevis oocytes (stage-I). A two-color home-made microscope has been used for this investigation. In particular, a 785nm Raman probe has been used to acquire the spontaneous Raman scattering from the oocyte cytoplasm, while a 532nm probe has been employed to detect ...
RUSCIANO, GIULIA +6 more
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Journal of Molecular Biology, 1973
Abstract Ribosomal RNA synthesized by hybrid frogs from the cross between Xenopus laevis and Xenopus mulleri was analyzed by molecular hybridization with purified ribosomal DNA from each species. Although the 18 S and 28 S rRNA sequences are indistinguishable between these two species, the remaining 10% of the 40 S rRNA precursor molecule of each ...
T, Honjo, R H, Reeder
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Abstract Ribosomal RNA synthesized by hybrid frogs from the cross between Xenopus laevis and Xenopus mulleri was analyzed by molecular hybridization with purified ribosomal DNA from each species. Although the 18 S and 28 S rRNA sequences are indistinguishable between these two species, the remaining 10% of the 40 S rRNA precursor molecule of each ...
T, Honjo, R H, Reeder
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Alginate Coating of Xenopus laevis Embryos
Biotechnology Progress, 2000Xenopus laevis eggs were coated, immediately after squeeze-stripping and fertilization, with a thin layer ( approximately 50 microm) of film based on one of three different types of alginates which varied in their mannuronic/guluronic acid ratio. The alginate was cross-linked with either Ca or Ba ions at three different concentrations.
N, Kampf, C, Zohar, A, Nussinovitch
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BREEDING OF XENOPUS LAEVIS DAUDIN
Acta Endocrinologica, 1964ABSTRACT A method for breeding Xenopus laevis Daudin is reported. This method has proved reliable for regular and frequent breeding throughout the year.
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