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Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos [PDF]
Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols.
Marcin Wlizla, Marko E. Horb
exaly +3 more sources
LIM domain-containing proteins Zyxin and LPP localize to apical epithelial cell-cell junctions at regions of stable F-actin [PDF]
LIM domain-containing proteins, Zyxin and Lipoma-Preferred Partner (LPP), localize to sites where actin filaments are strained, including actin stress fibers, focal adhesions, and cell-cell junctions. Utilizing the Xenopus laevis embryonic epithelium and
Katherine K. Tjoelker +4 more
doaj +2 more sources
Zmym4 is required for early cranial gene expression and craniofacial cartilage formation
Introduction: The Six1 transcription factor plays important roles in the development of cranial sensory organs, and point mutations underlie craniofacial birth defects.
Karyn Jourdeuil +6 more
doaj +1 more source
Albino Xenopus laevis tadpoles prefer dark environments compared to wild type
Tadpoles display preferences for different environments but the sensory modalities that govern these choices are not well understood. Here, we examined light preferences and associated sensory mechanisms of albino and wild-type Xenopus laevis tadpoles ...
Grace T Adebogun +20 more
doaj +1 more source
Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics
Understanding the biochemistry of the cell requires measurement of all the molecules it produces. Single-cell proteomics recently became possible through advances in microanalytical sample preparation, separation by nano-flow liquid chromatography ...
Bowen Shen +8 more
doaj +1 more source
MicroRNAs regulate gene expression at post-transcriptional levels. Some of them appear to regulate brain development and are involved in neurodevelopmental disorders.
A. Alwin Prem Anand +2 more
doaj +1 more source
Age-associated DNA methylation changes in Xenopus frogs
Age-associated changes in DNA methylation have been characterized across various animals, but not yet in amphibians, which are of particular interest because they include widely studied model organisms.
Marco Morselli +5 more
doaj +1 more source
The TFIIH complex is required to establish and maintain mitotic chromosome structure
Condensins compact chromosomes to promote their equal segregation during mitosis, but the mechanism of condensin engagement with and action on chromatin is incompletely understood.
Julian Haase +5 more
doaj +1 more source
Rab7 is required for mesoderm patterning and gastrulation in Xenopus
Early embryogenesis requires tightly controlled temporal and spatial coordination of cellular behavior and signaling. Modulations are achieved at multiple levels, from cellular transcription to tissue-scale behavior.
Jennifer Kreis +2 more
doaj +1 more source
Activating 5-HT1B/D receptors with the agonist Zolmitriptan was previously shown to facilitate Xenopus retinal ganglion cell (RGC) axon extension from ectopic eye primordia transplanted to the ventral fin.
Petros Basakis +2 more
doaj +1 more source

