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Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes

2023
Fluorescent dyes are often used to observe transport mechanisms in plant vascular tissues. However, it has been technically challenging to apply fluorescent dyes on roots to monitor xylem transport in vivo. Here, we present a fast, noninvasive, and high-throughput protocol to monitor xylem transport in seedlings.
Bartusch, Kai Steffen; id_orcid0000-0003-2708-5545   +3 more
openaire   +3 more sources

A pericycle‐localized silicon transporter for efficient xylem loading in rice

New Phytologist, 2022
Summary Rice is able to accumulate high concentrations of silicon (Si) in the shoots, and this ability is required for the mitigation of abiotic and biotic stresses. Although transporters for Si uptake have been identified, a transporter for the xylem loading of Si has not been found.
Sheng Huang   +5 more
openaire   +2 more sources

Evolution of Water Transport and Xylem Structure

International Journal of Plant Sciences, 2003
Land plants need water to replace the evaporation that occurs while atmospheric CO2 is diffusing into photosynthetic tissue. The water‐for‐carbon exchange rate is poor, and evolutionary history indicates a progression of innovations for cheap water transport—beginning in order with capillary suction at cell walls, stomatal regulation, hydroids ...
openaire   +1 more source

Effect of leaf water status and xylem pH on metabolism of xylem-transported abscisic acid

Plant Growth Regulation, 1997
Metabolism and distribution of xylem-fed ABA were investigated in leaves of maize (Zea mays) and Commelina communis when water stress and xylem pH manipulation were applied. 3H-ABA was fed to excised leaves via the transpiration stream. Water stress was applied through either a previous soil-drying before leaves were excised, or a quick dehydration ...
Jianhua Zhang, Wensuo Jia, Da-Peng Zhang
openaire   +1 more source

Perspectives on the Biophysics of Xylem Transport

2005
This chapter provides a brief discussion on cohesion-tension theory. The chapter also focuses on factors that affect hydraulic resistance in plants. It addresses how sap is extruded into the empty tracheary element even though the free energy gradient from soil to xylem seems often to be in the wrong direction, how the element is hydraulically isolated
Pickard, William F., Melcher, Peter J.
openaire   +1 more source

[20] Xylem Transport

1989
Publisher Summary Xylem perfusion is the use of pressure or suction to bring about the flow of an artificial solution through xylem vessels in an excised root or shoot segment. The advantages of a perfusion system are control over the composition of the solution entering the xylem vessels, ability to bring hormones and specific inhibitors into direct
openaire   +1 more source

Water transport in xylem conduits with ring thickenings

Plant, Cell & Environment, 1996
ABSTRACTHelical or annular wall thickenings are not only present in protoxylem, but may also he a feature of the tracheids or vessel elements of secondary wood. The frequency of their occurrence tends to be a function of climatic factors and conduit diameter.
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Xylem and phloem transport of micronutrients

2001
Knowledge on xylem, and especially phloem transport of micronutrients is important in developing our capacity to enhance loading of micronutrients into developing grain (which has consequences for human nutrition), to increase nutrient-use efficiency through increased remobilisation and re-utilisation of nutrients, to effectively nourish seedlings in ...
openaire   +1 more source

Waterlogging affects nitrogen transport in the xylem of soybean

Plant Physiology and Biochemistry, 1999
Abstract Transfer of nodulated and non-nodulated plants grown in vermiculite to hydroponic culture without soil was used to study waterlogging and nitrogen transport in the xylem of soybean. Non-aeration, aeration or aeration with nitrogen gas were used to obtain different levels of oxygen in the culture solutions.
Mário Puiatti, Ladaslav Sodek
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Ontogenetic changes in potassium transport in xylem of tomato

Physiologia Plantarum, 1982
The influence of plant ontogeny on xylem exudate K+ concentrations and K+ transport to the shoot was studied in both nutrient‐solution and field‐grown tomato plants (Lycopersicon esculentum).K+ concentrations in xylem exudate from decapitated plants decreased during tomato plant development from a high of 12 mM to a low of 5 mM.
Irvin Widders, Oscar A. Lorenz
openaire   +1 more source

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