Results 101 to 110 of about 62,083 (203)

Enhancing Enzyme Activity With Mutation Combinations Guided by Few‐Shot Learning and Causal Inference

Angewandte Chemie, EarlyView.
We present an integrated workflow that predicts activity‐enhancing mutation combinations from minimal experimental data. By proposing in vivo unit yield (yield/expression) as a surrogate for kcat/Km through causal inference, and visualizing local activity landscape, it effectively guides product yield improvement. ABSTRACT Designing enzyme sequences to
Lin Guo, Xiaoguang Yan, Yali Lu, Shengxin Nie, Mingyue Ge, Yukun Li, Weiguo Li, Xiaochun Zhang, Dongmei Liang, Yihan Zhao, Hongxiao Tan, Xiling Chen, Shilong Fan, Yefeng Tang, Jianjun Qiao, Boxue Tian   +15 more
wiley   +2 more sources

Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex. [PDF]

Microbiol Spectr
Lamote B, Cremelie E, Demeyere K, De Groote P, Grimon D, Vanderstraeten J, Meyer E, De Mey M, Briers Y.   +8 more
europepmc   +1 more source

Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine. [PDF]

Sci Rep, 2021
Trentelman JJA, Tomás-Cortázar J, Knorr S, Barriales D, Hajdusek O, Sima R, Ersoz JI, Narasimhan S, Fikrig E, Nijhof AM, Anguita J, Hovius JW.   +11 more
europepmc   +1 more source

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Engineering Antibodies into Targeted Chimeras: From Recognition Modules to Programmable Degraders

Advanced Science, EarlyView.
Antibody can be engineered into diverse lysosome‐targeting chimeras that convert target recognition into active degradation. By coupling antibody binding with recruited receptors, encoded domains, conjugated ligands, or nanoparticle scaffolds, these antibody‐associated targeted chimeras (AbTACs) platforms direct extracellular and membrane proteins ...
Kaige Chen, Quanyin Hu
wiley   +1 more source

Oral Immunization with Yeast-Surface Display of SARS-CoV-2 Antigens in <i>Pichia pastoris</i> Induces Humoral Responses in BALB/C Mice. [PDF]

Infect Dis Rep
de Macêdo LS, Espinoza BCF, Invenção MDCV, Pinho SS, Leal LRS, Silva MEDS, Bandeira BMA, Novis PVS, Souza THDS, Maux JML, Silva Neto JDC, de Freitas AC, Silva AJD.   +12 more
europepmc   +1 more source

Evaluation of the yeast surface display system for screening of functional nanobodies. [PDF]

AMB Express, 2020
Kajiwara K, Aoki W, Ueda M.
europepmc   +1 more source

Delay of Cucumber Senescence and Life‐Extending Capabilities on Caenorhabditis elegans With the Postharvest Application of Sorbitol and Methyl Salicylate

AgriFood: Journal of Agricultural Products for Food, EarlyView.
Methyl salicylate and sorbitol were taken as coating agents. Caenorhabditis elegans was taken as an animal model. The coated agents were applied in C. elegans. The life span study in C. elegans proves the authenticity of MS and SL. MS and SL extended the post‐harvest life of cucumber for 14 days.
Chirayu H. Trivedi, Vajida Munshi, Kalpesh J. Mehta, Jitendriya Panigrahi   +3 more
wiley   +1 more source

A yeast surface display platform for screening of non-enzymatic protein secretion in Kluyveromyces lactis. [PDF]

Appl Microbiol Biotechnol
An J, Shang N, Liu W, Niu Y, Liang Q, Jiang J, Zheng Y.   +6 more
europepmc   +1 more source

Exploring G protein-coupled receptors and yeast surface display strategies for viral detection in baker's yeast: SARS-CoV-2 as a case study. [PDF]

FEMS Yeast Res, 2021
Maneira C, Bermejo PM, Pereira GAG, de Mello FDSB.   +3 more
europepmc   +1 more source