A Universal Protein Ladder for Standardization of Diverse FRET Assays
Advanced Science, EarlyView.Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith +5 more
wiley +1 more source
Unique epitope-antibody interactions in the intrinsically disordered proteoglycan-like domain of human carbonic anhydrase IX defined by high-resolution NMR combined with yeast surface display. [PDF]
MAbs, 2023 Ni F +10 more
europepmc +1 more source
Current Challenges of Transcription Compartmentalization Research
Advanced Science, EarlyView.Transcription factors, coactivators, and RNA polymerase II assemble into transcription compartments ranging from small, defined complexes to liquid‐like condensates. This review unifies these seemingly competing descriptions along a single continuum and asks what these compartments have been shown to do, and what they have not, revealing that the most ...
Thomas Quail, Sina Wittmann
wiley +1 more source
Untangling Inositol (Pyro)Phosphate Biology Through Emerging Technologies
Advanced Science, EarlyView.Inositol (pyro)phosphates represent a vital class of intracellular messengers that govern multiple biological processes. The last decade has experienced a surge of interest in the functions of this class of signaling molecules, primarily due to the introduction of several innovative analytical technologies.
Adolfo Saiardi +5 more
wiley +1 more source
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species. [PDF]
Protein Eng Des Sel, 2022 Heinzelman P, Greenhalgh JC, Romero PA.
europepmc +1 more source
Probing Electrocatalyst Design for Product Selectivity in CO2 Reduction
Advanced Science, EarlyView.Selective CO2 electroreduction is controlled by catalyst structure, dynamic surface reconstruction, adsorbate interactions, and reaction microenvironment. This review summarizes Cu‐ and non‐Cu‐based catalysts, single‐atom and molecular systems, degradation pathways, and operando/computational strategies for steering CO2RR toward hydrocarbons ...
Suvodeep Sen +5 more
wiley +1 more source
Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections. [PDF]
Protein Eng Des Sel, 2021 Lown PS +5 more
europepmc +1 more source
Herbicide Metabolic Resistance in Poaceae Plants via the GA‐GID1/DELLA‐DOF2‐P450s Module
Advanced Science, EarlyView.Plant hormone signaling modulates herbicide resistance in Echinochloa crus‐galli. The GA–DELLA–DOF2 cascade directly activates P450 detoxification genes in two Poaceae species, uncovering a core mechanism of metabolic herbicide resistance. ABSTRACT Barnyard grass (Echinochloa crus‐galli) is one of the world's most important weeds, and the evolution of ...
Junzhi Wang +6 more
wiley +1 more source
Differential Processing of a <i>Bacillus subtilis</i> GH5 Endoglucanase During Yeast Surface Display in <i>Saccharomyces cerevisiae</i>. [PDF]
MicroorganismsRíos-Alvarado J +10 more
europepmc +1 more source
Advanced Science, EarlyView.Our study identifies an Hnf4a‐driven super‐enhancer of hepatic Ahcy that mediates the protective effects of intermittent fasting against MASLD. Disruption of the Ahcy super‐enhancer reduces Ahcy expression and exacerbates lipid accumulation. This pathway prevents aberrant promoter hypermethylation by maintaining the SAM/SAH balance, as exemplified by ...
Huafeng Chen +6 more
wiley +1 more source