Results 161 to 170 of about 62,083 (203)

Protein Engineering by Yeast Surface Display

Journal of Visualized Experiments
Protein engineering enables the improvement of existing functions of a given protein or the generation of novel functions. One of the most widely used and versatile tools in the protein engineering field is yeast surface display, where a pool of randomized proteins is expressed on the surface of yeast.
Charlotte U, Zajc, Elise, Sylvander, Magdalena, Teufl, Michael W, Traxlmayr   +3 more
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The Use of Yeast Surface Display in Biofuel Cells

2015
Biofuel cells are electrochemical devices which convert chemical energy to electricity using biochemical pathways and redox enzymes. In enzymatic fuel cells purified redox enzymes catalyze the reactions in the anode and cathode compartments whereas in microbial fuel cells (MFCs) the entire metabolism of the microorganisms is exploited.
Alon Szczupak, Lital Alfonta
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Yeast surface display for screening combinatorial polypeptide libraries

Nature Biotechnology, 1997
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity.
E T, Boder, K D, Wittrup
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Yeast Surface Display for In Vitro Biosynthetic Pathway Reconstruction

ACS Synthetic Biology, 2021
The enzymes immobilized through yeast surface display (YSD) can be used in in vitro metabolic pathway reconstruction as alternatives to the enzymes isolated or purified through conventional biochemistry methods. They can be easily prepared by growing and collecting yeast cells harboring display constructs.
Biaobiao Luo, Moonsoo M. Jin, Xiaohua Li, Nokwanda P. Makunga, Xuebo Hu   +4 more
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Engineering Antibody Affinity by Yeast Surface Display

2004
Publisher Summary This chapter elaborates the engineering antibody affinity by yeast surface display (YSD). YSD is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. The methods for displaying an antibody on yeast, creating mutant libraries and sorting libraries for improved clones are ...
David W, Colby, Brenda A, Kellogg, Christilyn P, Graff, Yik A, Yeung, Jeffrey S, Swers, K Dane, Wittrup   +5 more
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Protein Engineering and Selection Using Yeast Surface Display

2015
Yeast surface display is a powerful technology for engineering a broad range of protein scaffolds. This protocol describes the process for de novo isolation of protein binders from large combinatorial libraries displayed on yeast by using magnetic bead separation followed by flow cytometry-based selection.
ANGELINI, Alessandro, Chen TF, De Picciotto S, Yang NJ, Tzeng A, Santos MS, Van Deventer JA, Traxlmayr MW, Wittrup KD   +8 more
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Applications of Yeast Cell-Surface Display in Bio-Refinery

Recent Patents on Biotechnology, 2010
The dependency on depleting natural resources is a challenge for energy security that can be potentially answered by bioenergy. Bioenergy is derived from starchy and lignocellulosic biomass in the form of bioethanol or from vegetable oils in the form of biodiesel fuel. The acid and enzymatic methods have been developed for the hydrolysis of biomass and
Akihiko, Kondo, Tsutomu, Tanaka, Tomohisa, Hasunuma, Chiaki, Ogino   +3 more
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Ligand Engineering Using Yeast Surface Display

2014
The genotype-phenotype linkage provided by display technologies enables efficient synthesis, analysis, and selection of combinatorial protein libraries. This approach tremendously expands the protein sequence space that can be efficiently evaluated for a selectable function.
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Yeast Surface Display Methodology for the Characterization of Food Allergens In Situ

2023
High throughput allergen characterization is often based on phage display technique which is limited by the constraints of a prokaryotic expression system such as potential loss of conformational epitopes and lack of post-translational modifications.
Milica, Popović, Marija, Gavrović-Jankulović   +1 more
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Isolating and engineering human antibodies using yeast surface display

Nature Protocols, 2006
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display. Single-chain antibody fragments (scFvs) are first isolated from an existing nonimmune human library displayed on the yeast surface using magnetic-activated cell sorting selection followed by selection
Ginger, Chao, Wai L, Lau, Benjamin J, Hackel, Stephen L, Sazinsky, Shaun M, Lippow, K Dane, Wittrup   +5 more
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