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Flow Cytometric Screening of Yeast Surface Display Libraries
2004A method to screen and isolate antigen specific clones from a library of single-chain antibodies expressed on the surface of yeast cells is presented. Two rounds of magnetic bead enrichment before flow cytometric sorting enables one to screen libraries of far greater diversity than can be screened by just flow cytometry. The strength of flow cytometric
Michael, Feldhaus, Robert, Siegel
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[Advances in the application of yeast surface display technology].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2023Yeast surface display (YSD) is a technology that fuses the exogenous target protein gene sequence with a specific vector gene sequence, followed by introduction into yeast cells. Subsequently, the target protein is expressed and localized on the yeast cell surface by using the intracellular protein transport mechanism of yeast cells, whereas the most ...
Lili, Zhao +4 more
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The use of scFv-displaying yeast in mammalian cell surface selections
Journal of Immunological Methods, 2005Yeast surface display has proven to be a powerful tool for the directed evolution of immunological proteins when soluble ligands are available (Cho, B.K., Kieke, M.C., Boder, E.T., Wittrup, K.D., Kranz, D.M., 1998. A yeast surface display system for the discovery of ligands that trigger cell activation. J. Immunol.
Xin Xiang, Wang, Eric V, Shusta
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Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display
2022Yeast surface display (YSD) has been extensively used for protein design, engineering, and directed evolution in the past two decades. Here, we describe methods for directed evolution of tissue inhibitors of metalloproteinase (TIMP), the natural inhibitors of matrix metalloproteinases (MMPs), through design and generation of a combinatorial library of ...
Mari R, Toumaian +1 more
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Epitope-Specific Binder Design by Yeast Surface Display
2015Yeast surface display is commonly used to engineer affinity and design novel molecular interaction. By alternating positive and negative selections, yeast display can be used to engineer binders that specifically interact with the target protein at a defined site.
Jasdeep K, Mann, Sheldon, Park
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The contribution of Pir protein family to yeast cell surface display
Applied Microbiology and Biotechnology, 2014Proteins with internal repeats (Pir) in the Baker's yeast are located on the cell wall and include four highly homologous members. Recently, Pir proteins have become increasingly used as anchor proteins in yeast cell surface display systems. These display systems are classified into three types: N-terminal fusion, C-terminal fusion, and inserted fusion.
Na, Yang +7 more
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Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display
2012Although single-domain antibodies derived from libraries prepared either after animal immunization or naïve animals generally exhibit reasonable affinity, it is often desirable to further improve their affinity. This chapter describes protocols for improving the affinity of single-domain antibodies using quantitative library sorting by yeast surface ...
Akiko, Koide, Shohei, Koide
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Spacer-mediated display of active lipase on the yeast cell surface
Applied Microbiology and Biotechnology, 2001We have constructed a Saccharomyces cerevisiae strain displaying an active lipase on the cell surface by cell surface engineering. The gene encoding Rhizopus oryzae lipase (ROL) was fused with the genes encoding the pre-alpha-factor leader sequence and the C-terminal half of alpha-agglutinin including the glycosylphosphatidylinositol-anchor attachment ...
M, Washida +3 more
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Rapid Generation of Chicken Immune Libraries for Yeast Surface Display
2019Fluorescence-activated cell sorting (FACS) in combination with yeast surface display has emerged as a vital tool for the isolation and engineering of antibodies and antibody-derived fragments from synthetic, naïve, and immune libraries. However, the generation of antibodies against certain human antigens from immunized animals, e.g., mice, can remain ...
Jan P, Bogen +4 more
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Surface display of proteins in yeasts
2014Surface display of proteins in bacteria and yeasts has proved to be an interesting method of protein immobilization for different purposes. Generally, proteins of interest are genetically engineered by fusing them to autochthonous cell wall proteins, or parts of autochthonous proteins directing them to the wall and anchoring them at the cell surface ...
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