Results 81 to 90 of about 3,621,615 (290)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Threshold‐based classification of rapeseed using multi‐spectral vegetation indices from Sentinel‐2

open access: yesAgrosystems, Geosciences & Environment
Accurate mapping of rapeseed (Brassica napus) fields is critical for effective crop management, pollinator support, and yield forecasting. This study systematically evaluated the performance of 23 vegetation indices (VIs) for rapeseed detection during ...
Ehsan Rahimi, Chuleui Jung
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Index 1938 [PDF]

open access: yes, 1938
This is the 1938 edition of the Index. The Index is written by student editors. The Index is the college’s yearbook, it includes an annual record of events at Wooster. It includes the students, professors, student organizations, and athletics.
Editors, Index
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Index 1952 [PDF]

open access: yes, 1952
This is the 1952 edition of the Index. The Index is written by student editors, and it is the college’s yearbook. It includes an annual record of events at Wooster. This specific Index celebrates the fiftieth anniversary of the college.
Index Editors,
core  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Index 1941 [PDF]

open access: yes, 1941
This is the 1941 edition of the College of Wooster\u27s Index. The Index is written by student editors, and it is the college’s yearbook, it includes an annual record of events at Wooster. This specific edition begins with quiz questions, and the answers
Editors, Index
core   +1 more source

Index 2004 [PDF]

open access: yes, 2004
This is the 2004 edition of the Index. The Index is written by student editors, and it is the college’s yearbook. It includes an annual record of events at Wooster.
Editors, Index
core  

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