Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang +6 more
wiley +1 more source
Replacing Antibiotics with Synergistic Probiotics-Microalgae Consortium in Mud Crab (<i>Scylla paramamosain</i>) Larviculture: Transcriptomic Evidence for Enhanced Innate Immunity, Oxidative Stress Response, and Metabolic Adaptability. [PDF]
Lv X +7 more
europepmc +1 more source
Pentatricopeptide repeat proteins coordinate nucleus‐organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA ...
Mingming Wu +7 more
wiley +1 more source
Influence of temperature on developmental and biochemical traits of the red squat lobster <i>Grimothea monodon</i> (H. Milne Edwards, 1837) during early ontogeny. [PDF]
Quispe-Machaca M +3 more
europepmc +1 more source
The transcriptional adapter ADA2 interacts with the histone acetyltransferase GCN5 to regulate starch and storage protein content in wheat grains through histone acetylation. ADA2 undergoes phase separation and is modulated by GCN5, affecting histone acetyltransferase activity and gene transcription.
Xiaobang Zhang +15 more
wiley +1 more source
Keystone active bacterial lineages associated with Penaeus stylirostris shrimp health across larvae stages. [PDF]
Callac N +7 more
europepmc +1 more source
PanGraphRNA is an easy‐to‐use platform that improves RNA‐seq analysis by using graph pangenomes instead of a single reference genome. It increases read alignment accuracy, detects important genes missed by traditional methods, and works across major crops, helping researchers obtain more reliable transcriptome results for diverse plant research and ...
Yifan Bu +10 more
wiley +1 more source
Potential Bacterial Biomarkers Associated with <i>Penaeus stylirostris</i> Shrimp Larvae to Infer Holobiont Health and Dysbiosis Across Larvae Stages. [PDF]
Callac N +7 more
europepmc +1 more source
Towards an integrated molecular understanding of plant hormones
This review offers a comprehensive overview of the nine plant hormones, delving into their biosynthesis, transport, signaling and crosstalk mechanisms. Because the complexity of plant hormonal control goes beyond these core elements, additional specific features are discussed. Lastly, this review highlights how fundamental insights drive hormonal‐based
Louise Vilain +2 more
wiley +1 more source
Acute toxicity of local anesthetics used for humans and other animals in the larvae of marine crustacean, red-clawed crab Chiromantes haematocheir. [PDF]
Nishikawa A +17 more
europepmc +1 more source

