Results 61 to 70 of about 5,464 (253)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Zooming in on ZooMS: New Approaches to ZooMS Protocols and Further Insights into Animal Identifications in Neolithic and Bronze Age Indus Valley Sites

, 2023
Method Development of the ZooMS protocol to increase success rate of taxonomic identification of heavily degraded faunal materials.
Sebastian Millien
core   +1 more source

Editorial: To zoom or not to zoom – that is the question [PDF]

International Journal of Social Psychiatry, 2021
Sam Nishanth Gnanapragasam, Keith Hariman, Antonio Ventriglio   +2 more
openaire   +3 more sources

Zooming into the pandemic! A forensic analysis of the Zoom Application

Forensic Science International: Digital Investigation, 2021
The global pandemic of COVID-19 has turned the spotlight on video conferencing applications like never before. In this critical time, applications such as Zoom have experienced a surge in its user base jump over the 300 million daily mark (ZoomBlog, 2020).
Andrew Mahr, Meghan Cichon, Sophia Mateo, Cinthya Grajeda, Ibrahim M. Baggili   +4 more
openaire   +3 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Solving the two-decades-old murder case through joint application of ZooMS and ancient DNA approaches [PDF]

, 2023
Bones are one of the most common biological types of evidence in forensic cases. Discriminating human bones from irrelevant species is important for the identification of victims; however, the highly degraded bones could be undiagnostic morphologically ...
Wang, N., Li, C., Jiang, H., Cui, Y., Gao, S., Ma, P., Zhang, Q., Xu, Y., Yang, S., Shi, S., Wu, Y.   +10 more
core   +1 more source

A new legacy: potential of zooarchaeology by mass spectrometry in the analysis of North American megafaunal remains

Frontiers in Mammal Science
Museum legacy collections, often derived from large-scale archaeological excavations, can serve as paleoenvironmental archives of Late Pleistocene megafaunal composition and dynamics. Many of these collections, however, contain large quantities of highly
Mariya Antonosyan, Eden Hill, Margaret Jodry, Noel Amano, Samantha Brown, Torben Rick, Nicole Boivin, Nicole Boivin, Nicole Boivin   +8 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Improving elasmobranch baselines in the California Channel Islands through ZooMS and stable isotope analyses

Frontiers in Environmental Archaeology
Sharks are among the world's most endangered vertebrates, having declined by over 70% in abundance over the past 50 years—a collapse driven primarily by modern fishing. However, the extent of this impact is difficult to assess without improved records of
Michael Buckley, Emma A. Elliott Smith, Emma A. Elliott Smith, Ellie-May Oldfield, Clara Boulanger, Clara Boulanger, Andrew C. Kitchener, Andrew C. Kitchener, Francisco J. Concha, Torben C. Rick, Todd J. Braje   +10 more
doaj   +1 more source