Results 11 to 20 of about 82,842 (255)

Diagnosis of quinolone-resistant Coxiella burnetii strains by PCR-RFLP [PDF]

Journal of Clinical Laboratory Analysis, 2000
A total of 12 strains of Coxiella burnetii (8 Greek isolates from acute Q-fever patients, two reference strains-Nine Mile and Q212-and two pefloxacin-resistant laboratory strains) were examined for the presence of point mutations in the quinolone resistance determining region (QRDR) of gyrA gene by direct DNA sequencing of the polymerase chain reaction
Anna Psaroulaki, Yannis Tselentis, Ioanna Spyridaki, Ana M. Aransay, Efstathia Scoulica   +4 more
openaire   +4 more sources

Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex. [PDF]

Parasit Vectors, 2020
Background: Chromosomal inversion polymorphisms play a role in adaptation to heterogeneous environments. Inversion polymorphisms are implicated in the very high ecological flexibility of the three main malaria vector species of the Afrotropical Anopheles
Montanez-Gonzalez R, Pichler V, Calzetta M, Love RR, Vallera A, Schaecher L, Caputo B, Pombi M, Petrarca V, Della Torre A, Besansky NJ.   +10 more
europepmc   +3 more sources

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum [PDF]

, 2008
BACKGROUND: Sulphadoxine-pyrimethamine has been abandoned as first- or second-line treatment by most African malaria endemic countries in favour of artemisinin-based combination treatments, but the drug is still used as intermittent preventive treatment ...
AO Talisuna, AP Alker, C Wongsrichanalai, CH Sibley, Chantal van Overmeir, CV Plowe, DG Altman, FO ter Kuile, G Dorsey, J Chaparro, J Le Bras, Jean-Claude Dujardin, JJ Juliano, Maryline Bonnet, MK Laufer, MT Duraisingh, P Ouma, PE Wilson, Petra F Mens, RA Cardullo, S Cohuet, S Decuypere, T Triglia, Umberto d'Alessandro, World Health Organization Regional Office for Africa, World Health Organization Regional Office for Africa   +25 more
core   +14 more sources

Identification of Eutypa lata by PCR-RFLP [PDF]

Plant Disease, 2004
Eutypa lata is a vascular canker pathogen of woody plants commonly diagnosed by isolating the pathogen from infected tissue. Related fungi from the same family, the Diatrypaceae, also have been found in association with grapevine in Californian vineyards. An in situ polymerase chain reaction (PCR) method has been developed for detection of E.
Walter D. Gubler, Florent P. Trouillas, Philippe E. Rolshausen   +2 more
openaire   +2 more sources

تمرکز ایزوالکتریک و روشهای متصل به PCR-RFLP برای کشف کمبود Alpha1-antitrypsin [PDF]

Archives of Razi Institute, 2004
53 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF ...
ب.غ. گودرزی, ا. لطفی, ا. مصباح, ا. زارع میرک آبادی, ر. باقریان   +4 more
doaj   +1 more source

Identification of the Mutation in DCLRE1C Gene by PCR-RFLP

Selcuk Tip Dergisi, 2023
Amaç: DCLRE1C genindeki mutasyonlar Artemis proteininin fonksiyonel olarak bozulmasına neden olur ve T/B hücre gelişimi olumsuz etkilenir. Bu mutasyonun bir sonucu olarak, genellikle ağır kombine ve kombine immün yetmezlik (CID) kliniği ortaya çıkar. Akraba evliliğinin yaygın olduğu bölgemizde bu mutasyona bağlı CID vakalarına sıklıkla rastlanmaktadır.
Mehmet Ali Karaselek, Serkan Kuccukturk, Hasan Kapakli, Esra Hazar, Sukru Nail Guner, Sevgi Keles, Ercan Kurar, Ismail Reisli   +7 more
openaire   +2 more sources

Characterization of Ascaris from Ecuador and Zanzibar [PDF]

, 2014
To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (
A.M. Sparks, Arizono, Betson, C. Sandoval, Draper, G. Oviedo, J.R. Stothard, M. Betson, P.J. Cooper, Takata   +9 more
core   +3 more sources

PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil

Electronic Journal of Biotechnology, 2015
Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses.
Diego Rayan Teixeira de Sousa, Carla Silvana da Silva Santos, Bodo Wanke, Roberto Moreira da Silva Júnior, Marcelo Cordeiro dos Santos, Kátia Santana Cruz, Rossicléia Lins Monte, Andreas Nocker, João Vicente Braga de Souza   +8 more
doaj   +1 more source

PCR‐RFLP typing of the bovine myoglobin gene

Animal Genetics, 1994
SummaryWe have identified substitutions in the 31 untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi‐allelic restriction fragment length polymorphism. Co‐dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction—restriction ...
Agaba, Morris, Kemp, Stephen J.
openaire   +4 more sources

PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) Polimorfismos PCR-RAPD e PCR-RFLP detectados em Anopheles cruzii (Diptera, Culicidae)

Revista Brasileira de Entomologia, 2006
The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa ...
Daniéla Calado, Mario Antonio Navarro-Silva, Maria Anice Mureb Sallum   +2 more
doaj   +1 more source