Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina.
semanticscholar +1 more source
Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold+225 more
core +3 more sources
Derivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis
Background The analysis of cellulose molecular weight distributions by gel permeation chromatography (GPC) is a powerful tool to obtain detailed information on enzymatic cellulose hydrolysis, supporting the development of economically viable biorefinery ...
Engel Philip, Hein Lea, Spiess Antje C
doaj +1 more source
Identification of novel components of Trypanosoma brucei editosomes [PDF]
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole+6 more
core +2 more sources
Determination of Dioxins and Dioxin-like Polychlorinated Biphenyls in Pork by Gas Chromatography-Tandem Mass Spectrometry [PDF]
A gas chromatography-tandem mass spectrometry (GC-MS/MS) with stable isotope dilution (SID) method was established for the determination of dioxins (PCDD/Fs) and dioxin like polychlorinated biphenyls (DL-PCBs) in pork. The sample was extracted by Soxhlet
LU Jing, JIAO Yanna, CHENG Jing, SHEN Tiantian, ZHU Shaohua, FU Shanliang
doaj +1 more source
Gel chromatographic characterization of immunoreactive adrenocorticotropin in patients with ACTH hypersecretion [PDF]
We investigated the molecular size of circulating immunoreactive ACTH by gel chromatography in patients with ACTH hypersecretion due to various disorders of the hypothalamic-pituitary-adrenal axis.
A Chatelain+19 more
core +1 more source
Separation of single-walled carbon nanotubes with a gel permeation chromatography system.
A gel permeation chromatography system is used to separate aqueous sodium dodecyl sulfate suspensions of single-walled carbon nanotubes (SWCNTs). This automated procedure requires no precentrifugation, is scalable, and is found to yield monochiral SWCNT ...
B. Flavel+4 more
semanticscholar +1 more source
Obtaining Of β-Lactoglobulin By Gel Filtration Of Cow Milk Whey [PDF]
Milk whey proteins carry out a number of important biological functions and also they are precursors of many biologically active peptides (antihypertensive peptides, antagonists of opioid receptors, regulators of intestinal motility, immunomodulatory ...
Datsyshyn, K. (Kateryna)+2 more
core +3 more sources
A database was developed to store, organize, and retrieve the data associated with electrophoresis and chromatography separations. It allows laboratories to store extensive data on separation techniques (analytical and preparative).
Kenneth D. Cole
doaj +1 more source
Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques [PDF]
The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml.
Nawal Thnoon Youis+2 more
doaj +1 more source