Results 21 to 30 of about 42,771 (161)

Machine Learning Combined with Restriction Enzyme Mining Assists in the Design of Multi-Point Mutagenic Primers

Mathematics, 2022
The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and high specificity; thus, many small and medium laboratories use it to
Yu-Huei Cheng, Li-Yeh Chuang, Cheng-Hong Yang   +2 more
doaj   +1 more source

Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP and RFLP. [PDF]

PLoS ONE, 2016
BACKGROUND:The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid
Wen-Lin Liu, Huei-Chuan Shih, I-Szu Weng, Ya-Zhu Ko, Chi-Chu Tsai, Chang-Hung Chou, Yu-Chung Chiang   +6 more
doaj   +1 more source

‘Candidatus Phytoplasma solani’: From infection to all‐round characterisation of distinct tuf‐type strains with different virulence in tomato plants

Annals of Applied Biology, EarlyView.
‘Candidatus Phytoplasma (Ca. P.) solani’ is associated with bois noir (BN) of grapevine and stolbur of solanaceous plants and is primarily transmitted by Hyalesthes obsoletus Signoret. Four tuf‐a and five tuf‐b1 ‘Ca. P. solani’ strains were transmitted to tomato plants (cv. Micro‐Tom) by using bait‐plants and by capturing H.
Gaia Carminati, Francesco Pavan, Chiara Bernardini, Diana Sommaro, Abderraouf Sadallah, Rita Musetti, Paolo Ermacora, Marta Martini   +7 more
wiley   +1 more source

Evaluating Associations Between Ankylosing Spondylitis, Torque Teno Virus and Polymorphisms in Interleukin 6 and Vitamin D Receptor Genes

International Journal of Immunogenetics, EarlyView.
ABSTRACT The etiology of ankylosing spondylitis (AS) is complex and not yet fully understood. Interleukin‐6 (IL‐6), vitamin D and the vitamin D receptor (VDR) play an important role in modulating immune response, and Torque teno virus is considered a marker of immune status. This case–control study aimed to investigate the predisposition to AS. A total
Dănuț Cimponeriu, Lavinia‐Mariana Berca, Irina Radu, Mihai Toma, Remus Nica, Silvia Nica, Roxana Măciucă   +6 more
wiley   +1 more source

Molecular Testing in Sickle Cell Disease: From Newborn Screening to Transfusion Care

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Sickle cell disease (SCD) is one of the most frequent monogenic diseases worldwide and a highly heterogeneous and complex disease. SCD care carries several challenges. This includes early and accurate diagnosis as well as optimal red blood cell transfusion matching in this population carrying a high risk of alloimmunization.
Thomas Pincez, Yves D. Pastore
wiley   +1 more source

Molecular identification of Candida species isolated from cases of neonatal candidemia using polymerase chain reaction-restriction fragment length polymorphism in a tertiary care hospital

Indian Journal of Pathology and Microbiology, 2017
Context: Candida spp. is an emerging cause of bloodstream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs in various Candida species are responsible for the increase in morbidity ...
Akeela Fatima, Gulnaz Bashir, Tehmeena Wani, Abiroo Jan, Amrish Kohli, Mosin S Khan   +5 more
doaj   +1 more source

PCR‐RFLP typing of the bovine myoglobin gene

Animal Genetics, 1994
SummaryWe have identified substitutions in the 31 untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi‐allelic restriction fragment length polymorphism. Co‐dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction—restriction ...
Agaba, Morris, Kemp, Stephen J.
openaire   +3 more sources

Plastid Engineering for Photosynthesis‐Driven Synthesis of Hyaluronic Acid in Tobacco

Plant Biotechnology Journal, EarlyView.
ABSTRACT Hyaluronic acid (HA) is a glycosaminoglycan composed of alternating units of N‐acetylglucosamine and glucuronic acid. High moisture retention, viscoelasticity and biocompatibility are unique features that make HA polymers attractive compounds for medical applications and aesthetic purposes.
Amanda Lopes, Omar Sandoval‐Ibáñez, Stéphanie Arrivault, David Rolo, F. Vanessa Loiacono, Alexander Erban, Daniel Karcher, Stephan Obst, Stephanie Ruf, Joachim Kopka, Ralph Bock   +10 more
wiley   +1 more source

Harnessing Bulk‐Segregant Mapping to Identify Trait‐Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Forward genetics has been instrumental in identifying genes underlying desirable traits, yet its application to polyploid plants, many of which are key agricultural crops, remains challenging due to their genomic complexity. Therefore, we developed BenthMap, a bulk segregant analysis platform for high‐throughput trait mapping and gene ...
Zuba Ahmed, Jiyuan An, Satomi Hayashi, Julia Bally, Chris Winefield, Peter M. Waterhouse   +5 more
wiley   +1 more source

Is Micronucleus Assay a Suitable Biomarker for Evaluating the Cancer Risk in Professionals Exposed to Antineoplastic Drugs? A Systematic Review

Journal of Applied Toxicology, Volume 46, Issue 3, Page 733-753, March 2026.
ABSTRACT The widespread use of antineoplastic drugs in cancer treatment has led to significant concerns regarding the potential health risks posed to healthcare professionals involved in the preparation, administration, and handling of these chemical compounds, including genotoxicity.
Thiago Guedes Pinto, Lorrany da Silva Avanci, Gabriel Carvalhal de Aguiar, Daniel Vitor de Souza, Patricia Ramos Cury, Ana Claudia Muniz Renno, Daniel Araki Ribeiro   +6 more
wiley   +1 more source