Results 21 to 30 of about 82,842 (255)

Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP

Journal of Pharmacopuncture, 2009
Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences ...
Ki-Rok Kwon, Seung-Il Baek, Suk Ho Choi
doaj   +1 more source

Typing class I HLA-A gene using a nested PCR-RFLP procedure

Brazilian Journal of Medical and Biological Research, 2005
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution ...
E.C. Castelli, D.S. Gil, L.C.S. Veiga, J.L.V. de Camargo   +3 more
doaj   +1 more source

Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts) [PDF]

, 2005
Apolipoprotein E (apoE) is a multifunctional circulating 34-kDa protein, whose gene encodes single-nucleotide polymorphisms linked to several neurodegenerative diseases.
Dickson, JG, Owen, JS, Simons, JP, Tagalakis, AD   +3 more
core   +1 more source

Infection of Gammarus duebeni populations by two vertically transmitted microsporidia; parasite detection and discrimination by PCR–RFLP [PDF]

, 2002
We screened a population of the brackish water crustacean Gammarus duebeni from the Isle of Cumbrae for the presence of vertically transmitted microsporidia.
Dunn, A.M., Hatcher, M.J., Hogg, J.C., Ironside, J.E., Sharpe, R.G., Smith, J.E.   +5 more
core   +1 more source

Métodos subsidiários para o diagnóstico da Leishmaniose tegumentar americana (LTA): comparação dos resultados do seqüenciamento de DNA e da PCR-RFLP para determinação da espécie de leishmania em amostras cutâneo-mucosas Subsidiary methods for the diagnosis of American tegumentar leishmaniasis (ATL): comparison of sequencing of DNA and PCR-RFLP for identification of leishmania species in skin samples

Anais Brasileiros de Dermatologia, 2005
FUNDAMENTOS: Métodos moleculares têm-se mostrados mais eficazes para o diagnóstico da LTA. OBJETIVOS: Comparar os resultados da intradermorreação de Montenegro (IRM), presença de leishmania em biópsia (Bc), reação de imunofluorescência indireta (Rifi ...
Flávio C. Barbosa Garcia, Sandra Silva Rodrigues dos Santos, Maria Fernanda Chociay, Ângela C. Rapela Medeiros, Ana Maria F. Roselino   +4 more
doaj   +1 more source

Animal Species Identification by PCR – RFLP of Cytochrome b

Scientific Papers Animal Science and Biotechnologies, 2023
An alternative DNA detection system is based on the polymerase chain reaction (PCR) amplification of a segment of the mitochondrial cytochrome b gene.
Tomáš Minarovič, Anna Trakovická, Alica Rafayová, Zuzana Lieskovská   +3 more
doaj  

Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages

Czech Journal of Food Sciences, 2017
The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in
Ivona Djurkin Kušec, Danijela Samac, Vladimir Margeta, Žarko Radišić, Dragutin Vincek, Goran Kušec   +5 more
doaj   +1 more source

Genes do eixo somatotrófico e características de crescimento numa população F2 de bovinos Somatotrophic axis genes on growth traits in a bovine F2 population

Pesquisa Agropecuária Brasileira, 2006
O objetivo deste trabalho foi avaliar a associação dos polimorfismos dos genes bGH, IGF-1 e PIT-1 com características de peso e ganho de peso numa população F2 de bovinos (Gir x Holandês), pela técnica de PCR-RFLP.
Marcos Vinicius Gualberto Barbosa da Silva, Mário Luiz Martinez, Marco Antonio Machado, Carlos Souza do Nascimento, Ana Lúcia Campos, Marta Fonseca Martins Guimarães, Ana Luisa Sousa Azevedo, Antônia Kécya França Moita, Jeffrey Frederico Lui   +8 more
doaj   +1 more source

Symbiont diversity is not involved in depth acclimation in the Mediterranean sea whip Eunicella singularis [PDF]

, 2011
In symbiotic cnidarians, acclimation to depth and lower irradiance can involve physiological changes in the photosynthetic dinoflagellate endosymbiont, such as increased chlorophyll content, or qualitative modifications in the symbiont population in ...
Allemand, D., Caligara, C., Cerrano, C., Ciosi, M., Forcioli, D., Francour, P., Merle, P.L., Muti, C.   +7 more
core   +2 more sources

Identification of nine species of the Chlamydiaceae using PCR-RFLP [PDF]

International Journal of Systematic and Evolutionary Microbiology, 1999
The family Chlamydiaceae contains two genera and nine species. Rapid and easy identification of these species is essential for taxonomic, epidemiological and clinical determinations. Currently, DNA sequence analysis is the only accepted method that decisively distinguishes all nine species.
Karin D. E. Everett, Arthur A. Andersen
openaire   +3 more sources